Suc-Ala-Phe-Lys-AMC is a highly sensitive fluorogenic substrate. Suc-Ala-Phe-Lys-AMC can be specifically recognized and cleaved by plasmin, releasing the AMC fluorophore. Enzyme activity is quantified by measuring the change in fluorescence intensity. Suc-Ala-Phe-Lys-AMC can be used for the detection and related research of plasmin and gingipain K[1-2].
References:
[1] Götz MG, James KE, Hansell E, et al. A new class of potent and selective inhibitors of asparaginyl endopeptidases (legumains) from evolutionarily diverse pathogens. J Med Chem. 2008 May 8;51(9):2816-32.
Suc-Ala-Phe-Lys-AMC是一种高度敏感的荧光底物。Suc-Ala-Phe-Lys-AMC可被纤溶酶特异性识别并切割，释放AMC荧光基团，通过检测荧光强度变化定量分析酶活性，Suc-Ala-Phe-Lys-AMC可用于纤溶酶和牙龈蛋白酶K的检测及相关研究[1]。