Hippuryl-Arg-OH is a fluorescent signal substrate used in enzyme activity assays for the identification and cleavage of peptide bonds containing arginine. Hippuryl-Arg-OH can be used for enzyme activity studies such as carboxypeptidase B and aminopeptidase, as well as related research in the field of drug development[1-4].
References:
[1] Liu YH, Konermann L. Enzyme conformational dynamics during catalysis and in the 'resting state' monitored by hydrogen/deuterium exchange mass spectrometry. FEBS Lett. 2006 Oct 2;580(22):5137-42.
[2] Fricker LD, Devi L. Comparison of a spectrophotometric, a fluorometric, and a novel radiometric assay for carboxypeptidase E (EC 3.4.17.10) and other carboxypeptidase B-like enzymes. Anal Biochem. 1990 Jan;184(1):21-7.
[3] Song L, Fricker LD. Purification and characterization of carboxypeptidase D, a novel carboxypeptidase E-like enzyme, from bovine pituitary. J Biol Chem. 1995 Oct 20;270(42):25007-13.
[4] Casarini DE, Alves KB, Araújo MS, et al. Endopeptidase and carboxypeptidase activities in human urine which hydrolyze bradykinin. Braz J Med Biol Res. 1992;25(3):219-29.
Hippuryl-Arg-OH是一种用于识别和切割含有精氨酸的肽键的酶活性测定的荧光信号底物。Hippuryl-Arg-OH可用于羧肽酶B、氨肽酶等酶活性研究以及药物研发领域的相关研究[1-4]。