Tetraglycine (H-Gly-Gly-Gly-Gly-OH) is an oligopeptide composed of four glycine monomers[1]. Tetraglycine can be used as a "flexible spacer/linker" in NHS ester coupling reactions[2].
In vitro, The EC50 value for the intracellular calcium ion response of NCI-H716 cells to Tetraglycine is 3mM. Application of 10mM Tetraglycine induces membrane depolarization. 15min of treatment with 10mM Tetraglycine does not affect the increase in intracellular cAMP formation. 2h of treatment with 10mM Tetraglycine promotes GLP-1 secretion from cells[3].
In vivo, Tetraglycine used as a "metabolism and transport probe". The plasma half-lives of Tetraglycine is about 5min. Tetraglycinecan be absorbed and accumulated by tissues, especially the kidneys[2].
References:
[1] Adibi SA, Morse EL. Enrichment of glycine pool in plasma and tissues by glycine, di-, tri-, and tetraglycine. Am J Physiol. 1982;243(5):E413-E417.
[2] Khan MA, Ghanim RW, Kiser MR, et al. Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers. Nanomaterials (Basel). 2022;12(4):608.
[3] Le Nevé B, Daniel H. Selected tetrapeptides lead to a GLP-1 release from the human enteroendocrine cell line NCI-H716. Regul Pept. 2011;167(1):14-20.
Tetraglycine(H-Gly-Gly-Gly-Gly-OH)是一种由四个甘氨酸单体组成的寡肽[1]。Tetraglycine可用作NHS酯偶联反应中的“柔性间隔物/连接剂”[2]。
体外实验中，Tetraglycine对NCI-H716胞内钙离子反应的EC50值为3mM。10mM Tetraglycine可诱导NCI-H716膜去极化。10mM Tetraglycine处理15分钟不影响NCI-H716胞内cAMP的生成。10mM Tetraglycine处理2小时可促进NCI-H716分泌GLP-1[3]。
体内实验中，Tetraglycine可用作“代谢和转运探针”。Tetraglycine的血浆半衰期约为5分钟。Tetraglycine会被组织吸收和积累，特别是肾脏组织[2]。