Tetraglycine (H-Gly-Gly-Gly-Gly-OH) 是一种由四个甘氨酸单体组成的寡肽。
Cas No.:637-84-3
Sample solution is provided at 25 µL, 10mM.
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Tetraglycine (H-Gly-Gly-Gly-Gly-OH) is an oligopeptide composed of four glycine monomers[1]. Tetraglycine can be used as a "flexible spacer/linker" in NHS ester coupling reactions[2].
In vitro, The EC50 value for the intracellular calcium ion response of NCI-H716 cells to Tetraglycine is 3mM. Application of 10mM Tetraglycine induces membrane depolarization. 15min of treatment with 10mM Tetraglycine does not affect the increase in intracellular cAMP formation. 2h of treatment with 10mM Tetraglycine promotes GLP-1 secretion from cells[3].
In vivo, Tetraglycine used as a "metabolism and transport probe". The plasma half-lives of Tetraglycine is about 5min. Tetraglycinecan be absorbed and accumulated by tissues, especially the kidneys[2].
References:
[1] Adibi SA, Morse EL. Enrichment of glycine pool in plasma and tissues by glycine, di-, tri-, and tetraglycine. Am J Physiol. 1982;243(5):E413-E417.
[2] Khan MA, Ghanim RW, Kiser MR, et al. Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers. Nanomaterials (Basel). 2022;12(4):608.
[3] Le Nevé B, Daniel H. Selected tetrapeptides lead to a GLP-1 release from the human enteroendocrine cell line NCI-H716. Regul Pept. 2011;167(1):14-20.
Tetraglycine(H-Gly-Gly-Gly-Gly-OH)是一种由四个甘氨酸单体组成的寡肽[1]。Tetraglycine可用作NHS酯偶联反应中的“柔性间隔物/连接剂”[2]。
体外实验中，Tetraglycine对NCI-H716胞内钙离子反应的EC50值为3mM。10mM Tetraglycine可诱导NCI-H716膜去极化。10mM Tetraglycine处理15分钟不影响NCI-H716胞内cAMP的生成。10mM Tetraglycine处理2小时可促进NCI-H716分泌GLP-1[3]。
体内实验中，Tetraglycine可用作“代谢和转运探针”。Tetraglycine的血浆半衰期约为5分钟。Tetraglycine会被组织吸收和积累，特别是肾脏组织[2]。
| Cell experiment [1]: | |
Cell lines | NCI-H716 cells |
Preparation Method | Cells were exposed to a Tetraglycine (H-Gly-Gly-Gly-Gly-OH) solution, followed by measurement of cell membrane potential using DIBAC3(5), and detection of intracellular cAMP and GLP-1 levels. |
Reaction Conditions | 10mM; 20mM; 15min; 2h |
Applications | Application of 10mM Tetraglycine induced membrane depolarization. 15min of treatment with 10mM Tetraglycine did not affect the increase in intracellular cAMP formation. 2h of treatment with 20mM Tetraglycine promoted GLP-1 secretion from cells. |
| Animal experiment [2]: | |
Animal models | Male Sprague-Dawley rats; 270-300g |
Preparation Method | The right jugular vein was injected with one of the following test solutions: 150mM NaCl, 300mM Glycine, 150mM Diglycine, 100mM Triglycine, or 75mM Tetraglycine. Plasma glutathione concentrations were measured at 2, 5, 10, 15, 20, and 30 minutes after injection. The concentrations of glutathione in the liver, muscle, and kidney were measured at 5 and 30 minutes after injection. |
Dosage form | 1pM/g; i.v. |
Applications | Two minutes after the injection of glycine, there was over a tenfold increase in plasma glycine concentration. Each increase in the number of glycine residues resulted in further reduction in the initial rise in plasma glycine concentration. The plasma half-lives of all these peptides are remarkably similar and are about 5min. They were absorbed by the organization. Injection of Triglycine and Tetraglycine resulted in greater glycine concentration in the kidney than injection of either Glycine or Diglycine. Cach increase in the number of glycine residues resulted in greater recovery of glycine peptides from the kidney. |
References: | |
| Cas No. | 637-84-3 | SDF | |
| 别名 | 四聚甘胺酸 | ||
| 分子式 | C8H14N4O5 | 分子量 | 246.22 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0614 mL | 20.307 mL | 40.6141 mL |
| 5 mM | 812.3 μL | 4.0614 mL | 8.1228 mL |
| 10 mM | 406.1 μL | 2.0307 mL | 4.0614 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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2.
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