Glutaryl-Gly-Arg-AMC is a fluorescent substrate and a peptide substrate of urokinase plasminogen activator (uPA). The maximum excitation/emission wavelength of the fluorophore AMC is 380nm/460nm[1, 2]. Glutaryl-Gly-Arg-AMC can be used to detect protease activity, especially trypsin and trypsin-like enzymes[3, 4].
References:
[1] Wu G, Mazzitelli B A, Quek A J, et al. Tranexamic acid is an active site inhibitor of urokinase plasminogen activator[J]. Blood advances, 2019, 3(5): 729-733.
[2] Dewey S. Fluctuating Effects of Diabetes Mellitus Type 1 and 2 on the Ubiquitin Proteasome System in Two Rodent Models: Proteasome Inhibition is Not the Magic Answer[M]. University of California, Davis, 2014.
[3] Xu J H, Jiang Z, Solania A, et al. A commensal dipeptidyl aminopeptidase with specificity for N-terminal glycine degrades human-produced antimicrobial peptides in vitro[J]. ACS chemical biology, 2018, 13(9): 2513-2521.
[4] Maffioli E, Jiang Z, Nonnis S, et al. High-resolution mass spectrometry-based approaches for the detection and quantification of peptidase activity in plasma[J]. Molecules, 2020, 25(18): 4071.
Glutaryl-Gly-Arg-AMC是一种荧光底物，是尿激酶纤溶酶原激活剂（uPA）的肽底物，荧光团AMC的最大激发/发射波长为380nm/460nm[1, 2]。Glutaryl-Gly-Arg-AMC能够用于检测蛋白酶活性，特别是胰蛋白酶（Trypsin）和类胰蛋白酶（Trypsin-like enzymes）[3, 4]。