Cyclolinopeptide B is a cyclic nonapeptide with significant biological activity [1]. Cyclolinopeptide B inhibits the activation of T cells and B cells and downregulates the expression of immune-related genes (such as IL-2 and IFN-γ) [2]. Cyclolinopeptide B is commonly used to treat cancer [3].
In Sk-Br-3 cells, Cyclolinopeptide B (25, 50, 100, 200, 400μg/mL; 24h, 48h) has concentration dependent cytotoxicity [4]. In human gastric SGC7901 cells, Cyclolinopeptide B (40, 80, 120, 160, 200, 240μM; 24h, 48h) induces apoptosis by activating Fas, FasL, caspase 8, and caspase 3 [5]. In human gastric SGC7901 cells, Cyclolinopeptide B (80, 120, 200μM; 24h) isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway [6].
References:
[1]. Morita H, Shishido A, Matsumoto T, et al. A new immunosuppressive cyclic nonapeptide, cyclolinopeptide B from Linum usitatissimum. Bioorganic & Medicinal Chemistry Letters. 1997 May 20; 7(10): 1269-1272.
[2]. Aladedunye F, Sosinska E, Przybylski R. Flaxseed cyclolinopeptides: analysis and storage stability. Journal of the American Oil Chemists' Society. 2013 Mar; 90: 419-428.
[3]. Fojnica A, Gromilic Z, Vranic S, et al. Anticancer potential of the cyclolinopeptides. Cancers. 2023 Jul 30; 15(15): 3874.
[4]. Okinyo-Owiti DP, Dong Q, Ling B, et al. Evaluating the cytotoxicity of flaxseed orbitides for potential cancer treatment. Toxicology reports. 2015 Jan 1; 2: 1014-1018.
[5]. Zou XG, Hu JN, Li J, et al. iCellular uptake of [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed, and their antitumor activities in human gastric SGC-7901 cells. Journal of Functional Foods. 2018 Sep 1; 48: 692-703.
[6]. Zou XG, Hu JN, Wang M, et al. [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway. Journal of Functional Foods. 2019 Jan 1; 52: 332-339.
Cyclolinopeptide B是一种具有显著生物活性的环状九肽 [1]。Cyclolinopeptide B抑制T细胞和B细胞的活化，并下调免疫相关基因（如IL-2和IFN-γ）的表达 [2]。Cyclolinopeptide B常用于治疗癌症 [3]。
在Sk-Br-3细胞中，Cyclolinopeptide B（25、50、100、200、400μg/mL；24h、48h）具有浓度依赖性细胞毒性 [4]。在人胃癌SGC7901细胞中，Cyclolinopeptide B（40、80、120、160、200、240μM；24h、48h）通过激活Fas、FasL、caspase 8和caspase 3诱导细胞凋亡 [5]。在人胃SGC7901细胞中，从亚麻籽中分离的Cyclolinopeptide B（80、120、200μM；24h）通过调节AKT/JNK信号通路诱导SGC-7901细胞G1细胞周期停滞 [6]。