Amyloid β-Protein (16-20)

Amyloid β-Protein (16-20) is a five-amino acid fragment of the amyloid-β protein. Amyloid β-Protein (16-20) disrupts the formation of β-sheet structures by competitively binding to the hydrophobic core region of the Aβ protein^[1-2]. Amyloid β-Protein (16-20) can be used in research related to the pathological mechanisms of Alzheimer's disease and drug screening^[3-4].

In vitro, Amyloid β-Protein (16-20) (100nM) was applied to rat striatal and hippocampal synaptosomes for 1 hour. Amyloid β-Protein (16-20) did not increase the percentage of apoptotic signals nor alter the survival rate of the synaptosomes^[5]. Amyloid β-Protein (16-20) (100µg/mL) was used to treat differentiated human SH-SY5Y neuroblastoma cells (induced by retinoic acid and brain-derived neurotrophic factor) overnight, resulting in a small amount of cell death. Treatment of human red blood cells with Amyloid β-Protein (16-20) (100µg/mL) for 3 hours caused hemolysis^[6].

References:
[1] Chandra Saha P, Das RS, Chatterjee T, et al. Supramolecular β-Sheet Forming Peptide Conjugated with Near-Infrared Chromophore for Selective Targeting, Imaging, and Dysfunction of Mitochondria. Bioconjug Chem. 2020 May 20;31(5):1301-1306.
[2] Waugh ML, Wolf LM, Moore KA, et al. Rationally Designed Peptoid Inhibitors of Amyloid-β Oligomerization. Chembiochem. 2024 Jul 2;25(13):e202400060.
[3] Kino R, Araya T, Arai T,et al. Covalent modifier-type aggregation inhibitor of amyloid-β based on a cyclo-KLVFF motif. Bioorg Med Chem Lett. 2015 Aug 1;25(15):2972-5.
[4] Niu L, Liu L, Xi W, et al. Synergistic Inhibitory Effect of Peptide-Organic Coassemblies on Amyloid Aggregation. ACS Nano. 2016 Apr 26;10(4):4143-53.
[5] Trebesova H, Olivero G, Marchi M, et al. The Anti-Aggregative Peptide KLVFF Mimics Aβ1-40 in the Modulation of Nicotinic Receptors: Implications for Peptide-Based Therapy. Biomedicines. 2022 Sep 8;10(9):2231.
[6] Roytrakul S, Jaresitthikunchai J, Phaonakrop N, et al. Secretomic changes of amyloid beta peptides on Alzheimer's disease related proteins in differentiated human SH-SY5Y neuroblastoma cells. PeerJ. 2024 Jul 17;12:e17732.

Amyloid β-Protein (16-20)是一种β-淀粉样蛋白的五个氨基酸片段。Amyloid β-Protein (16-20)通过竞争性结合Aβ蛋白的疏水核心区域来破坏β-折叠结构的形成^[1-2]。Amyloid β-Protein (16-20)可用于阿尔茨海默病病理机制和药物筛选的相关研究^[3-4]。

在体外，Amyloid β-Protein (16-20)（100nM）大鼠纹状体及海马突触小体1小时。Amyloid β-Protein (16-20)未增加凋亡信号百分比，未改变突触小体的存活率^[5]。Amyloid β-Protein (16-20)（100μg/mL）过夜处理经维甲酸和脑源性神经营养因子诱导分化的人SH-SY5Y神经母细胞瘤细胞，可导致少量的细胞死亡；Amyloid β-Protein (16-20)（100μg/mL）处理人红细胞3小时，可导致的溶血^[6]。