Abz-Ala-Phe-Arg-Phe-Ser-Gln-EDDnp is the synthetic substrate of human kallikrein 6 (KLK6), with kcat/Km = 38667s⁻¹mM⁻¹[1]. Abz-Ala-Phe-Arg-Phe-Ser-Gln-EDDnp is widely used as an internal quenching peptide, and after incubation with KLK6, the hydrolysis reaction can be monitored at the excitation wavelength of 320nm and the emission wavelength of 420nm[2].
References:
[1] Angelo P F, Lima A R, Alves F M, et al. Substrate specificity of human kallikrein 6: salt and glycosaminoglycan activation effects[J]. Journal of Biological Chemistry, 2006, 281(6): 3116-3126.
[2] Dos Santos J F, Borçari N R, da Silva Araújo M, et al. Mesenchymal stem cells differentiate into keratinocytes and express epidermal kallikreins: Towards an in vitro model of human epidermis[J]. Journal of cellular biochemistry, 2019, 120(8): 13141-13155.
Abz-Ala-Phe-Arg-Phe-Ser-Gln-EDDnp是human kallikrein 6(KLK6)的合成底物，kcat/Km值为38667s-1mM-1[1]。Abz-Ala-Phe-Arg-Phe-Ser-Gln-EDDnp被广泛用作内猝灭肽，在与KLK6孵育后，可在激发波长320nm和发射波长420nm处监测水解反应[2]。