Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate.
V8 protease (0, 0.1, 0.5, 1.0, 5.0, 10, 20 µg/ml) is assayed with a solution of 200 µM of the synthetic substrate (Fluorescent Substrate for Glu-Specific Proteases; ABz-AFAFEVFY(NO2)D-OH) in HEPES buffer pH 7.4 and at room temperature conditions. The fluorescence was read each 1 min for 60 min by fluorescence microplate reader[1].
References:
[1]. Qudsi, et al. Development of a novel approach to influence the secretion of pathogenicity factors ofStaphylococcus aureusby synthetic peptides. Fr., 04. Mai. 2012.