Fluorescein-12-UTP, 10mM Sodium solution, a fluorescently labeled nucleotide analog that serves as a substrate for RNA polymerases such as SP6, T3, and T7, is widely used for RNA labeling and detection, with an Ex/Em of 496nm/520nm[1,2]. In in vitro transcription reactions, Fluorescein-12-UTP, 10 mM Sodium solution can replace uridine triphosphate (UTP) and be incorporated into RNA via an amide bond, thereby enabling fluorescent labeling of RNA. Fluorescein-12-UTP, 10mM Sodium solution is suitable for applications such as cellular fluorescence imaging, fluorescence in situ hybridization (FISH), bioimaging, and diagnostics[3,4,5].
References:
[1] CLARKE P A. Labeling and purification of RNA synthesized by in vitro transcription[M]//RNA-Protein Interaction Protocols. Totowa, NJ: Humana Press, 1999: 1-10.
[2] SEETHALA R. Homogeneous assays for high-throughput and ultrahigh-throughput screening[M]//Handbook of Drug Screening. New York: CRC Press, 2001: 90-149.
[3] HUGHES S C, DREAN B S L, LIVNE-BAR I, et al. Fluorescence in situ hybridization in whole-mount Drosophila embryos[J]. Biotechniques, 1996, 20(5): 748-750.
[4] YANG L, ELLINGTON A D. 2 Prospects for the De Novo Design of Nucleic Acid[J]. Fluorescence Sensors and Biosensors, 2005: 5.
[5] BELLINGHAM J, CHAURASIA S S, MELYAN Z, et al. Evolution of melanopsin photoreceptors: discovery and characterization of a new melanopsin in nonmammalian vertebrates[J]. PLoS Biology, 2006, 4(8): e254.
Fluorescein-12-UTP, 10mM Sodium solution是一种可作为SP6、T3和T7等RNA聚合酶底物的荧光标记核苷酸类似物，被广泛用于RNA标记和检测，Ex/Em为496nm/520nm[1,2]。Fluorescein-12-UTP, 10mM Sodium solution在体外转录反应中可替代尿苷三磷酸（UTP），通过酰胺键与尿苷三磷酸结合，从而实现对RNA的荧光标记，适用于细胞荧光成像、原位杂交（FISH）和生物成像与诊断等领域[3,4,5]。