Fibrinogen (Bovine) is a soluble plasma glycoprotein synthesized in hepatic parenchymal cells with a molecular weight of approximately 340kDa[1]. Fibrinogen (Bovine) can be converted into fibrin by thrombin and plays a core role in the coagulation process. Its structure is highly similar to human fibrinogen and is widely used in biomedical research[2]. Fibrinogen (Bovine) can be used as an extracellular matrix (ECM) protein to promote osteogenic differentiation of human mesenchymal stem cells (hMSC)[3]. Fibrinogen (Bovine) can enhance the proliferation and survival of centroblast cell lines[4]. Fibrinogen (Bovine) can induce endothelial cell permeability, regulate the activation of NF-κB in endothelial cells, and upregulate the expression of monocyte chemoattractant protein-1 (MCP-1) and IL-8 mRNA[5, 6].
References:
[1] Meredith S. Biotechnology for detecting oxidative modifications to fibrinogen[D]. Aston University, 2016.
[2] Weisel J W. Fibrinogen and fibrin[J]. Advances in protein chemistry, 2005, 70: 247-299.
[3] Linsley C, Wu B, Tawil B. The effect of fibrinogen, collagen type I, and fibronectin on mesenchymal stem cell growth and differentiation into osteoblasts[J]. Tissue Engineering Part A, 2013, 19(11-12): 1416-1423.
[4] Lefevre E A, Hein W R, Stamataki Z, et al. Fibrinogen is localized on dark zone follicular dendritic cells in vivo and enhances the proliferation and survival of a centroblastic cell line in vitro[J]. Journal of Leucocyte Biology, 2007, 82(3): 666-677.
[5] Tyagi N, Roberts A M, Dean W L, et al. Fibrinogen induces endothelial cell permeability[J]. Molecular and cellular biochemistry, 2008, 307: 13-22.
[6] Guo M, Sahni S K, Sahni A, et al. Fibrinogen regulates the expression of inflammatory chemokines through NF-κB activation of endothelial cells[J]. Thrombosis and haemostasis, 2004, 92(10): 858-866.
Fibrinogen (Bovine)是一种在肝实质细胞中合成的可溶性血浆糖蛋白，分子量约为340kDa[1]。Fibrinogen (Bovine)能够被凝血酶转化为纤维蛋白，在凝血过程中发挥核心作用，结构与人类纤维蛋白原高度相似，被广泛应用于生物医学研究[2]。Fibrinogen (Bovine)能够用作细胞外基质（ECM）蛋白促进人间充质干细胞（hMSC）成骨分化[3]。Fibrinogen (Bovine)能够增强中心母细胞系的增殖和存活[4]。Fibrinogen (Bovine)能够诱导内皮细胞通透性，还可以调节内皮细胞中NF-κB的激活，并上调单核细胞趋化蛋白-1（MCP-1）和IL-8 mRNA的表达[5, 6]。