Ferrichrome (iron-free)是一种来自Ustilago sphaerogena的铁离子载体，可作为亚铁螯合酶的铁源。
Cas No.:34787-28-5
Sample solution is provided at 25 µL, 10mM.
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Ferrichrome (iron-free) is a ferric ionophore for Ustilago sphaerogena, which can serve as a source of iron for the enzyme ferrochelatase [1]. Ferrichrome can induce the adaptive growth of fission yeast on high ammonium medium and restore the iron-dependent pathways to compensate for the dysfunction of the vacuoles induced by ammonium [2]. Ferrichrome has been widely used to induce the iron transporter Arn1p from the endosomal pathway to the plasma membrane pathway in Saccharomyces cerevisiae[3].
In vitro, Ferrichrome treatment for 24 hours significantly inhibited HT29 cells, SKCO1 cells, and SW480 cells, with IC50 values of 0.02µg/ml, 0.34µg/ml, and 0.38µg/ml, respectively[4]. Treatment with 100µg/ml Ferrichrome for 72 hours significantly induced apoptosis in MKN-45 cells, accompanied by an increase in the expression levels of cleaved PARP and cleaved caspase-9[5]. Treatment with 10µg/ml Ferrichrome for 48 hours significantly increased the expression level of DDIT3 mRNA in SW620 cells, stimulated the activity of the JNK signaling pathway, and promoted cell death[6].
In vivo, Ferrichrome treatment via intraperitoneal injection at a dose of 10mg/kg/day for 8days significantly reduced tumor growth in mice with SUIT-2 xenografts[7].
References:
[1] Straka J G, Emergy T. The role of ferrichrome reductase in iron metabolism of Ustilago sphaerogena[J]. Biochimica et Biophysica Acta (BBA)-Enzymology, 1979, 569(2): 277-286.
[2] Chiu P C, Nakamura Y, Nishimura S, et al. Ferrichrome, a fungal-type siderophore, confers high ammonium tolerance to fission yeast[J]. Scientific reports, 2022, 12(1): 17411.
[3] Kim Y, Yun C W, Philpott C C. Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae[J]. The EMBO Journal, 2002, 21(14): 3632-3642.
[4] Iwama T, Fujiya M, Konishi H, et al. Bacteria‐derived ferrichrome inhibits tumor progression in sporadic colorectal neoplasms and colitis‐associated cancer[J]. Cancer cell international, 2021, 21(1): 21.
[5] Ijiri M, Fujiya M, Konishi H, et al. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells[J]. Tumor Biology, 2017, 39(6): 1010428317711311.
[6] Konishi H, Fujiya M, Tanaka H, et al. Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis[J]. Nature communications, 2016, 7(1): 12365.
[7] Kita A, Fujiya M, Konishi H, et al. Probiotic-derived ferrichrome inhibits the growth of refractory pancreatic cancer cells[J]. International journal of oncology, 2020, 57(3): 721-732.
Ferrichrome (iron-free)是一种来自Ustilago sphaerogena的铁离子载体，可作为亚铁螯合酶的铁源[1]。Ferrichrome可诱导裂殖酵母在高铵培养基上的适应性生长，并恢复依赖铁的途径以补偿铵诱导的液泡功能障碍[2]。Ferrichrome已被广泛用于诱导酿酒酵母中铁转运蛋白Arn1p从内吞途径向质膜途径的转运[3]。
在体外，Ferrichrome处理HT29细胞、SKCO1细胞和SW480细胞24小时，显著抑制了细胞活力，IC50值分别为0.02µg/ml、0.34µg/ml和0.38µg/ml[4]。100µg/ml的Ferrichrome处理MKN-45细胞72小时，显著诱导了细胞凋亡，同时伴随着cleaved PARP和cleaved caspase-9表达水平的升高[5]。10µg/ml的Ferrichrome处理SW620细胞48小时，显著增加了DDIT3 mRNA的表达水平，刺激了JNK信号通路的活性，并促进了细胞死亡[6]。
在体内，每日腹腔注射10mg/kg剂量的Ferrichrome，连续8天，显著减少了携带 SUIT-2异种移植瘤小鼠的肿瘤生长[7]。
| Cell experiment [1]: | |
Cell lines | SW480 cells |
Preparation Method | SW480 cells were seeded in 96-well plates at a density of 1×104 cells in high-glucose DMEM medium with 10% (v/v) fetal bovine serum (FBS), 2mM L-glutamine, 50U/ml penicillin, and 50µg/ml streptomycin in a humidified atmosphere containing 5% CO2. After 24h of treatment with different concentrations of Ferrichrome (0, 0.1, 1, 2.5, and 5µg/ml), the cell viability was measured. |
Reaction Conditions | 0, 0.1, 1, 2.5, and 5µg/ml; 24h |
Applications | Ferrichrome treatment significantly inhibited the cell viability of SW480 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male BALB/c mice |
Preparation Method | Male BALB/c mice (6 weeks old; 22-25g) were housed singly in a standard environment with food and water ad libitum. SUIT-2 cells (1×106/0.1ml) were injected subcutaneously into the back of male BALB/c nude mice. PBS for the studies evaluating the effects of Ferrichrome compared with PBS, Ferrichrome (10mg/kg/day) treatment was intraperitoneally administered after the injection of SUIT-2 cells for 8 days. The tumor volume was analyzed. |
Dosage form | 10mg/kg/day for 8 days; i.p. |
Applications | Ferrichrome treatment significantly reduced tumor growth in mice with SUIT-2 xenografts. |
References: | |
| Cas No. | 34787-28-5 | SDF | |
| 别名 | Desferrichrome | ||
| 化学名 | cyclo(glycylglycylglycyl-N5-acetyl-N5-hydroxy-L-ornithyl-N5-acetyl-N5-hydroxy-L-ornithyl-N5-acetyl-N5-hydroxy-L-ornithyl) | ||
| Canonical SMILES | O=C([C@H](CCCN(C(C)=O)O)NC([C@H](CCCN(C(C)=O)O)NC(CN1)=O)=O)N[C@@H](CCCN(C(C)=O)O)C(NCC(NCC1=O)=O)=O | ||
| 分子式 | C27H45N9O12 | 分子量 | 687.7 |
| 溶解度 | Water: 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4541 mL | 7.2706 mL | 14.5412 mL |
| 5 mM | 290.8 μL | 1.4541 mL | 2.9082 mL |
| 10 mM | 145.4 μL | 727.1 μL | 1.4541 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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