GlpBio

Fast Red Violet LB

目录号: GC66319纯度: >98.00%
Fast Red Violet LB是一种偶氮染料，能够用于检测抗酒石酸酸性磷酸酶（TRAP）和碱性磷酸酶（ALP）。
Fast Red Violet LB
Cas No.: 32348-81-5
规格价格库存数量操作
50mg¥1,080.00现货
1
100mg¥1,710.00现货
1
250mg¥3,420.00现货
1
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文献被引

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产品描述 Description

Fast Red Violet LB is an azo dye that can be used to detect tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP)[1, 2]. Fast Red Violet LB, also known as carminic acid, is extracted from the scales of female cochineal insects[3]. Fast Red Violet LB can be used to study the role of TNF-α in fracture healing[4]. Fast Red Violet LB can stain naphthol AS-D chloroacetate esterase activity and can be used to detect granulocytes[5].

References:
[1] Tsuchiya T, Matsumoto Y, Kurihara S. The fluorescent simultaneous azo dye technique for demonstration of tartrate-resistant acid phosphatase (TRAP) activity in osteoclast-like multinucleate cells[J]. Journal of bone and mineral metabolism, 1995, 13: 71-76.
[2] Conn's biological stains: a handbook of dyes, stains and fluorochromes for use in biology and medicine[M]. Taylor & Francis, 2020.
[3] Baqri S S R. Dye-Yielding Insects[J]. Commercial Insects, 2023: 218-237.
[4] Gerstenfeld L C, Cho T J, Kon T, et al. Impaired fracture healing in the absence of TNF‐α signaling: The role of TNF‐α in endochondral cartilage resorption[J]. Journal of Bone and Mineral Research, 2003, 18(9): 1584-1592.
[5] Petrie-Hanson L, Ainsworth A J. Differential cytochemical staining characteristics of channel catfish leukocytes identify cell populations in lymphoid organs[J]. Veterinary Immunology and Immunopathology, 2000, 73(2): 129-144.

Fast Red Violet LB是一种偶氮染料，能够用于检测抗酒石酸酸性磷酸酶（TRAP）和碱性磷酸酶（ALP）[1, 2]。Fast Red Violet LB也称为胭脂红酸，是从雌性胭脂虫昆虫的鳞片中提取的[3]。Fast Red Violet LB能够被用于研究 TNF-α在骨折愈合过程中的作用[4]。Fast Red Violet LB能够对萘酚AS-D氯乙酸酯酶活性进行染色，能够用于检测粒细胞[5]。

实验参考方法 Experimental Reference Method

本方案仅提供一个指导，请根据您的具体需要进行修改。

1. 溶液配制

(1)工作液：用实验缓冲液配制Fast Red Violet LB染色液，配置浓度为0.6mg/mL。

注意：最佳的工作浓度请根据实际情况调整或参阅文献自行设置梯度浓度进行摸索。工作液必须现配现用。

2. 用Fast Red Violet LB进行抗酒石酸酸性磷酸酶（TRAP）染色(来自文献,仅做参考)

(1)细胞染色：将细胞用PBS中的4%多聚甲醛固定，然后用PBS洗涤，并用丙酮和乙醇（1：1）的混合物处理1min，再次用PBS洗涤，并在37°C下在含有0.2mg/mL萘酚AS-MX磷酸盐和0.6mg/mL Fast Red Violet LB的TRAP缓冲液（pH 5.2）中孵育30min。用纯水洗涤后，在显微镜下观察[1]。

(2)组织染色：将载玻片（股骨样品）在含有萘酚AS-MX磷酸盐、Fast Red Violet LB和MnCl2的乙酸钠缓冲液（0.1M，pH 5.0）中在酒石酸钠存在下于37 ℃孵育60min，用于TRAP染色[2]。

3. 用Fast Red Violet LB进行碱性磷酸酶（ALP）染色(来自文献,仅做参考)

(1) 细胞染色：细胞固定后用0.1mg/mL萘酚AS-MX磷酸盐和0.6mg/mL Fast Red Violet LB对细胞进行染色，然后用数码相机拍照[3]。

(2) 细胞3D培养物染色：用冷的10%中性福尔马林缓冲液固定样品15min。用dH2O冲洗后，在室温下，将样品与0.1mg/mL萘酚AS-MX磷酸盐底物（溶于0.4% N，N-二甲基甲酰胺和0.1M HCl，pH 8.3，含0.6mg/mL Fast Red Violet LB）孵育45min，以使产物可视化。染色液用dH2O洗去，在立体镜下观察，使用ImageJ软件通过测量每张图像中红色染色面积与总面积的百分比来定量ALP染色[4]。

References:

[1] Imai H, Yoshimura K, Miyamoto Y, et al. Roles of monocarboxylate transporter subtypes in promotion and suppression of osteoclast differentiation and survival on bone[J]. Scientific Reports, 2019, 9(1): 15608.

[2]Hamamura K, Hamajima K, Yo S, et al. Deletion of Gb3 synthase in mice resulted in the attenuation of bone formation via decrease in osteoblasts[J]. International journal of molecular sciences, 2019, 20(18): 4619.

[3] Yoo S H, Kim J G, Kim B S, et al. BST2 mediates osteoblast differentiation via the BMP2 signaling pathway in human alveolar-derived bone marrow stromal cells[J]. PloS one, 2016, 11(6): e0158481.

[4] Ioannidis K, Danalatos R I, Champeris Tsaniras S, et al. A custom ultra-low-cost 3D bioprinter supports cell growth and differentiation[J]. Frontiers in bioengineering and biotechnology, 2020, 8: 580889.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
32348-81-5
分子式
C14H11Cl2N3O·1/2ZnCl2
分子量
376.31 g/mol
保存条件
Store at -20°C,protect from light
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol