Ethylenediaminetetraacetic acid tetrasodium

Ethylenediaminetetraacetic acid (EDTA) tetrasodium is a kind of metal chelating agent (binds to bivalent and trivalent metal cations, including calcium). Ethylenediaminetetraacetic acid tetrasodium has antibacterial, anti-inflammatory, antioxidant, anti-hypercalcemia and anticoagulant activities. Ethylenediaminetetraacetic acid tetrasodium decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid tetrasodium can alleviate the liver fibrosis. Ethylenediaminetetraacetic acid tetrasodium can be used for coronary artery disease and neural system disease research.

Ethylenediaminetetraacetic acid has a strong bactericidal effect on the cell wall of P. aeruginosa and a. faecalis^[4].
Ethylenediaminetetraacetic acid (0.005-0.01 M) has good heavy metal extraction in contaminated silty-clay-loam soil columns, can extract Pb, Cd and Zn in a concentration-dependent way with an extraction efficiency sequence of Pb > Cd > Zn^[6].
Ethylenediaminetetraacetic acid (1.2 mM) enhances the activity of the CRE driving promoter by activating T-cell death-associated gene 8 (TDAG8) in HEK293T cells, thereby enhancing the production of cAMP in the cells^[7].

Ethylenediaminetetraacetic acid (60 mg/kg; Intraperitoneal injection; Three times per week for three weeks) can reduce liver fibrosis, lipid peroxidation and liver inflammation in CCl₄ induced liver fibrosis rats, and has antioxidant and anti-inflammatory activities^[5].

References:
[1]. Chumanov RS, et al. Artifact-inducing enrichment of ethylenediaminetetraacetic acid and ethyleneglycoltetraacetic acid on anion exchange resins. Anal Biochem. 2011 May 1;412(1):34-9.
[2]. Banfi G, et al. The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes. Clin Chem Lab Med. 2007;45(5):565-76.
[3]. Ibad A, et al. Chelation therapy in the treatment of cardiovascular diseases. J Clin Lipidol. 2016 Jan-Feb;10(1):58-62.
[4]. Gray GW, et al. The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria. J Gen Microbiol. 1965 Jun;39(3):385-99.
[5]. González-Cuevas J, et al. Ethylenediaminetetraacetic acid induces antioxidant and anti-inflammatory activities in experimental liver fibrosis. Redox Rep. 2011;16(2):62-70.
[6]. Naghipour D, et al. Remediation of heavy metals contaminated silty clay loam soil by column extraction with ethylenediaminetetraacetic acid and nitrilo triacetic acid[J]. Journal of Environmental Engineering, 2017, 143(8): 04017026.
[7]. Deai M, et al. Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells. Biochem Biophys Res Commun. 2022 Oct 20;626:15-20.