GlpBio

Erythrosin B (Erythrosin extra bluish)

目录号: GC33466纯度: >98.00%同义词: 赤藓红B,Erythrosin extra bluish
A red fluorescein dye and an inhibitor of dopamine reuptake
Erythrosin B (Erythrosin extra bluish)
Cas No.: 16423-68-0
规格价格库存数量操作
100mg¥446.00现货
1
10mM (in 1mL Water)¥491.00现货
1
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文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例
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    641, 529–536 (2025)
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    Science
    388(6745) (2025)
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    387(6734) (2025)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

产品描述 Description

Erythrosin B is a red fluorescein dye and an inhibitor of dopamine reuptake (IC50 = 45 ?M in rat brain synaptosomes).1,2,3 It has been used in the colorimetric and fluorescent determination of protein concentrations in vitro.1,2 Formulations containing erythrosine B have been used as color additives in food and pharmaceutical preparations.

1.Ma, C.Q., Li, K.A., and Tong, S.Y.Determination of proteins by fluorescence quenching of erythrosin BAnal. Chim. Acta333(1-2)83-88(1996) 2.Soedjak, H.S.Colorimetric micromethod for protein determination with erythrosin BAnal. Biochem.220(1)142-148(1994) 3.Lafferman, J.A., and Silbergeld, E.K.Erythrosin B inhibits dopamine transport in rat caudate synaptosomesScience205(4404)410-412(1979)

实验参考方法 Experimental Reference Method

Cell experiment:

The alkaline single-cell gel electrophoresis assay (comet assay) is performed. Briefly, 2×105 HepG2 cells are seeded in a 24-well plate for 24 h. The cells are then treated with Erythrosine B at 0.1, 0.2, 2.0, 10.0, 25.0, 50.0 or 70.0 μg/mL (final concentration) for 4 h; vehicle control (0.7% DMSO) and positive control (doxorubicin0.3 μg/mL) treatments are also performed. The HepG2 cell suspension is mixed with 37°C low-melting point agarose and transferred to normal-melting point agarose-coated slides. One hundred randomly chosen nucleoids are analyzed per treatment, and a total of three independent experiments are performed[1].

References:

[1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8.
[2]. Chequer FM, et al. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol. 2012 Oct;50(10):3447-51.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
16423-68-0
同义词
赤藓红B,Erythrosin extra bluish
SMILES
O=C1OC2(C3=C(OC4=C2C=C(I)C([O-])=C4I)C(I)=C([O-])C(I)=C3)C5=C1C=CC=C5.[Na+].[Na+]
分子式
C20H6I4Na2O5
分子量
879.86 g/mol
溶解性
Water : 20.7 mg/mL (23.53 mM)
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol