GlpBio

EPAC 5376753

目录号: GC31579纯度: >98%
COASDSData Sheet
EPAC5376753是Epac的变构调节抑制剂，在Swiss3T3细胞中抑制Epac1的IC50值为4µM。
EPAC 5376753
Cas No.: 302826-61-5
规格价格库存数量操作
250mg询价现货
1
500mg询价现货
1
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产品描述 Description

EPAC 5376753 is an allosterically inhibitor of Epac which inhibits Epac1 with an IC50 of 4 µM in Swiss 3T3 cells.

Epac is a key mediator of cAMP signaling. EPAC 5376753, the thiobarbituric acid modification of 5225554, has a greater ability to decrease the conformational changes of Epac necessary for its activation and to inhibit Epac signaling in cells. 5376753 selectively inhibits Epac-mediated migration in fibroblasts[1].

[1]. Brown LM, et al. Allosteric inhibition of Epac: computational modeling and experimental validation to identify allosteric sites and inhibitors. J Biol Chem. 2014 Oct 17;289(42):29148-57.

实验参考方法 Experimental Reference Method

Cell experiment:

Swiss 3T3 cells are treated with EPAC 5376753 (1, 10, 25, 50, 75, 100 μM) or 1% dimethyl sulfoxide for 48 h. Cell viability is assessed using the CellTiter-Glo assay[1].

References:

[1]. Brown LM, et al. Allosteric inhibition of Epac: computational modeling and experimental validation to identify allosteric sites and inhibitors. J Biol Chem. 2014 Oct 17;289(42):29148-57.

产品文档 Product Documents

Purity:>98%
COASDSData Sheet

化学性质Chemical Properties

CAS 号
302826-61-5
SMILES
O=C(/C(C(N1)=O)=C/C2=CC=C(C3=CC=C(Cl)C=C3Cl)O2)NC1=S
分子式
C15H8Cl2N2O3S
分子量
367.21 g/mol
溶解性
DMSO : 10 mg/mL (27.23 mM; ultrasonic and warming and heat to 60°C)
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol