Elastase from porcine pancreas (PPE), as one of the most prominent serine proteases,it can induce protease antiprotease imbalance and has been applied in an animal model of emphysema and Chronic pulmonary obstructive disease (COPD).It has 240 amino acids and a molecular weight of 26 kD. The conserved active site of elastase consists of three residues of serine 214, histidine 71, and aspartate 119 [1-3].
Elastase from porcine pancreas (0-60 mU/ml;24 h) treatment increased placenta growth factor (PlGF) expression and secretion in LE cells[4].
Elastase from porcine pancreas (100 mU/ml; weekly for one month;i.p) instillation increase PlGF expression, activation of JNK and p38 MAPK, and PlGF secretion in the pulmonary cells of mice[4]. Elastase from porcine pancreas can induces emphysema in hamsters[5].
References:
[1]. Shapiro SD. Animal models for COPD. Chest 2000; 117: 223S-227S.
[2]. M. WÜrtele, M. Hahn, K. Hilpert, W. HÖhne. Acta Crystallogr. D Biol. Crystallogr., 56 (2000), p. 520
[3]. Shotton DM, et, al. Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases. Nature. 1970 Feb 28;225(5235):802-6.
[4]. Hou HH, Cheng SL, et, al. Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor. Cell Death Dis. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329. PMID: 24008737; PMCID: PMC3789187.
[5]. Stone PJ, Lucey EC, et, al. Lnduction and exacerbation of emphysema in hamsters with human neutrophil elastase inactivated reversibly by a peptide boronic acid. Am Rev Respir Dis. 1990 Jan;141(1):47-52. doi: 10.1164/ajrccm/141.1.47. PMID: 2297186.
猪胰弹性蛋白酶Porcine pancreatic elastase (PPE)是最显著的丝氨酸蛋白酶之一,可以诱导蛋白酶抗蛋白酶失衡,并应用于肺气肿和慢性阻塞性肺疾病(COPD)的动物模型中。它有240个氨基酸和分子量为26 kD。弹性蛋白酶的保守活性位点由三个谷氨酸214、组氨酸71和天冬氨酸119残基组成[1-3]。
Elastase from porcine pancreas (0-60 mU/ml PPE;24 h)处理可以增加LE细胞中胎盘生长因子(PlGF)的表达和分泌[4]。
Elastase from porcine pancreas (100 mU/ml PPE; weekly for one month;i.p)可以增加小鼠肺细胞中PlGF的表达、JNK和p38 MAPK的活化以及PlGF的分泌[4]。Elastase from porcine pancreas可以在诱导仓鼠肺气肿[5]。