E235 is an expression regulator of activates transcription factor 4 (ATF4). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research[1].
E235 (1 µM；2、4、8、16 或 24 h) 以时间依赖性方式下调了 HT1080 细胞中 XBP-1s mRNA 的水平[1]。
E235 (0.1-10 µM；4 d 或 5 d) 对 HT1080、RPMI-8226、B16F10、4T1、HT1080 shNT 和 HT1080 shATF4 细胞具有抗增殖活性[1]。
E235 (0、1、5 和 10 µM；4 h) 以剂量依赖性方式增加了 AG1522 细胞中 p53 的表达。
E235 (1 µM；2 h) 增加了 AG1522 细胞中 p-Chk2 的表达[1]。
E235 (0、1、5 和 10 µM；2 h) 增加了 AG1522 和 HT1080 细胞中 p-p53 的表达[1]。
E235 (0、0.5、1、5 和 10 µM；0.5 h) 以剂量依赖性方式增加了 HT1080 细胞中 γ-H2AX 的表达[1]。
Western Blot Analysis[1]
| Cell Line: | HT1080, B16F10 and AG1522 cells. |
| Concentration: | 0, 1, 5 and 10 µM. |
| Incubation Time: | 4 h. |
| Result: | Increased the expression of ATF4 with dose-dependent manner. |
Western Blot Analysis[1]
| Cell Line: | HT1080 cells. |
| Concentration: | 0, 0.5, 1, 5 or 10 µM. |
| Incubation Time: | 2, 4 or 8 h. |
| Result: | Increased the expression of p-elF2α with time and dose dependent manner. |
Cell Proliferation Assay[1]
| Cell Line: | HT1080 or RPMI-8226 cells. |
| Concentration: | 1 µM. |
| Incubation Time: | 0, 8, 16 and 24 h. |
| Result: | Exhibited anti-proliferative activity. |
Cell Viability Assay[1]
| Cell Line: | AG1522 cells. |
| Concentration: | 0, 0.1, 0.5, 1 and 10 µM. |
| Incubation Time: | 4 d. |
| Result: | Inhibited cell viability with dose-dependent manner. |
Cell Cycle Analysis[1]
| Cell Line: | HT1080 cells and B16F10 cells. |
| Concentration: | 1 µM. |
| Incubation Time: | 8, 16 and 24 h. |
| Result: | Caused cell arrest during G2/M phase. |
[1]. Sayers CM, et al. Identification and characterization of a potent activator of p53-independent cellular senescence via a small-molecule screen for modifiers of the integrated stress response. Mol Pharmacol. 2013 Mar;83(3):594-604.