DSP, a homobifunctional cross-linker that reacts with amino groups such as lysine, and is widely used for surface functionalization, is the preferred linker for immobilizing enzymes and antibodies on gold surfaces[1-3]. DSP has a disulfide bridge within its structure, which cleaves upon binding to gold[2]. By immobilizing DSP on a gold thin film (GTF), dopamine (DA) can be selectively captured via the specific reaction between its primary amine and the NHS ester of DSP, enabling sensitive detection of DA in human cerebrospinal fluid (CSF) samples[4-5]. DSP can be employed in irreversible biotinylation procedures (IBP) to eliminate interference from intermolecular disulfide bonds during S-nitrosation detection[6]. DSP can also be used to isolate weak protein complexes and to prepare Au-TiO2 thin films for immunoassay experiments[3, 7].
References:
[1] Darder M, Takada K, Pariente F, et al. Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor. Anal Chem. 1999;71(24):5530-5537
[2] Ataman Sadık D, Boyacı İH, et al. Mixed monolayer decorated SPR sensing surface for thrombin detection. J Pharm Biomed Anal. 2019;176:112822.
[3] Akaki K, Mino T, Takeuchi O. DSP-crosslinking and Immunoprecipitation to Isolate Weak Protein Complex. Bio Protoc. 2022;12(15):e4478.
[4] Zhang K, Liu Y, Wang Y, et al. Quantitative SERS Detection of Dopamine in Cerebrospinal Fluid by Dual-Recognition-Induced Hot Spot Generation. ACS Appl Mater Interfaces. 2018;10(18):15388-15394.
[5] Gu H, Guo Y, Xiao X, et al. Double molecular recognition strategy based on boronic acid-diol and NHS ester-amine for selective electrochemical detection of cerebral dopamine. Anal Bioanal Chem. 2020;412(15):3727-3736.
[6] Huang B, Chen C. Detection of protein S-nitrosation using irreversible biotinylation procedures (IBP). Free Radic Biol Med. 2010;49(3):447-456.
[7] Meira DI, Barbosac AI, Proença M, et al. Immobilizing antibody biorecognition layers on Au–TiO2 thin films: direct (physisorption) vs. DSP-crosslinking (chemisorption) surface functionalization. Journal of Physics D: Applied Physics. 2024;415401.
DSP是一种与赖氨酸等氨基反应的同源双功能交联剂，广泛用于表面功能化，是将酶和抗体固定在金表面的首选连接分子[1-3]。DSP内含二硫键，可与金结合时断裂[2]。将DSP固定于金薄膜（GTF）后，可通过NHS酯与多巴胺（DA）伯胺的特异性反应选择性捕获DA，从而在人脑脊液（CSF）样本中实现高灵敏检测[4-5]。DSP可用于不可逆生物素化程序（IBP）处理中，排除分子间二硫键对S-亚硝基化检测的干扰[6]。DSP还可用于分离弱蛋白复合物及制备用于免疫分析实验的Au-TiO2薄膜[7-8]。