ANEPPS dye is a fast-response membrane potential dye.
Di-8-ANEPPS is a naphthyl styryl potential-sensitive fluorescent dye that can be used for non-invasive measurement of transmembrane voltage and responds quickly to changes in membrane potential[1]. Both the fluorescence excitation and emission spectra of Di-8-ANEPPS will change with the change of V m. Di-8-ANEPPS does not emit fluorescence in water, but it will emit strong fluorescence when combined with the lipid bilayer of the cell membrane, and the change of fluorescence intensity is proportional to the change of ΔΦ[2]. Di-8-ANEPPS has been used to detect transmembrane potentials in many types of static/dynamic cells, including nerve cells, endothelial cells, cardiac cells, and skeletal muscle cells.
Di-8-ANEPPS and Di-4-ANEPPS exhibit fairly consistent fluorescence intensity change rates (10% fluorescence change/100mV) in various tissues, cells and model membrane systems. Compared to Di-4-ANEPPS, Di-8-ANEPPS is more lipophilic and less susceptible to cellular internalization due to its sulfonate group, resulting in better retention on the plasma membrane, suitable for long-term membrane Potential research[2]. Di-8-ANEPPS is also more photostable and less toxic than Di-4-ANEPPS.
ANEPPS染料是快速响应的膜电位染料。
Di-8-ANEPPS是一种萘基苯乙烯基电位敏感荧光染料，可用于非侵入性测量跨膜电压，快速响应膜电位变化[1]。随着V m的变化，Di-8-ANEPPS的荧光激发和发射光谱都会发生变化。Di-8-ANEPPS在水中不发出荧光，但与细胞膜的脂质双层结合后会发出强烈荧光，且荧光强度的变化与ΔΦ的变化成正比[2]。Di-8-ANEPPS已被用于检测多种类型的静/动态细胞中的跨膜电位，包括神经细胞、内皮细胞、心脏细胞和骨骼肌细胞等。
Di-8-ANEPPS和Di-4-ANEPPS在各种组织，细胞和模型膜系统中表现出相当一致的荧光强度变化率(10%荧光变化/100mV)。相较于Di-4-ANEPPS，Di-8-ANEPPS更具亲脂性，并且由于具有磺酸盐基团而不容易受到细胞内化的影响，从而更好地保留在细胞质膜上，适合长期膜电位研究[2]。Di-8-ANEPPS 也比 Di-4-ANEPPS 更具有光稳定性和更低的毒性。
References:
[1]. Pucihar G, Kotnik T, and Miklavcic D. Measuring the induced membrane voltage with Di-8-ANEPPSJ. Vis. Exp.2009(33)(2016)
[2]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.