Di-4-ANEPPS is a fast-response membrane potential dye used to detect changes in membrane potential[1]. Di-4-ANEPPS fluoresces to potential changes through very rapid structural changes, so they are suitable for detecting fast and transient potential changes in cells. Di-4-ANEPPS is rapidly internalized in cells and is therefore mainly used in short-term experiments[2]. Di-4-ANEPPS is a highly water-soluble microinjection, the fluorescence is not affected by cell and tissue types, and can be used to measure membrane potential in various tissues and cell types as well as in artificial membranes. Di-4-ANEPPS is a common tool for mapping cardiac electrical activity[3].
Di-4-ANEPPS是一种快速响应的膜电位染料，用于检测膜电位变化[1]。Di-4-ANEPPS通过非常快速的结构变化对电位变化发出荧光，因此它们适合于检测细胞中快速和瞬态的电位变化。Di-4-ANEPPS在细胞中迅速内化，因此主要用于短期实验[2]。Di-4-ANEPPS是高度水溶性的微量注射剂，荧光不受细胞和组织类型的影响，能够用于测量各种组织和细胞类型以及人造膜中的膜电位。Di-4-ANEPPS是绘制心脏电活动的常用工具[3]。
References:
[1]. Maria Pura Hortigon-Vinagre, et al. The Use of Voltage Sensitive Dye di-4-ANEPPS and Video-Based Contractility Measurements to Assess Drug Effects on Excitation-Contraction Coupling in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. 2021 Mar 1;77(3):280-290. doi: 10.1097/FJC.0000000000000937.
[2]. LM Loew - Pure and Applied Chemistry. Potentiometric dyes: imaging electrical activity of cell membranes.1996 - degruyter.com
[3]. Marina Ronzhina, et al. Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart. 2021 Jun 10;12:667065. doi: 10.3389/fphys.2021.667065. eCollection 2021.