DBCO-PEG4-Biotin, also known as dibenzocyclooctyne polyethylene glycol biotin, is a versatile bioconjugation reagent[1]. DBCO can undergo a specific reaction with molecules containing azide groups to form stable covalent bonds through copper-free strain-promoted alkyne-azide cycloaddition (SPAAC)[2]. Therefore, DBCO-PEG4-Biotin can introduce biotin labeling into biomolecules via DBCO-PEG4-Biotin’s DBCO functional group.
References:
[1] Chen C, Zhao M, Guo J, Kuang X, Chen Z, Wang F. Electrochemical detection of FTO with N3-kethoxal labeling and MazF cleavage. RSC Adv. 2024 Aug 13;14(35):25561-25570.
[2] Hou Y, Arai S, Takei Y, Murata A, Takeoka S, Suzuki M. Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum. Sci Technol Adv Mater. 2016 Jul 18;17(1):293-299.
DBCO-PEG4-Biotin，也被称为二苯并环辛炔聚乙二醇生物素，是一种多功能的生物偶联试剂[1]。DBCO能够通过铜催化的炔-叠氮环加成反应（SPAAC）与含有叠氮基团的分子发生特异性反应，形成稳定的共价键[2]。因此，DBCO-PEG4-Biotin可通过DBCO官能团，在生物分子中引入生物素标记。