Dansyl chloride is a reactive probe for the derivatization of primary amines, including those on amino acids, peptides, and polyamines, for detection by HPLC.1,2,3 It has also been used for the derivatization of compounds containing phenol groups, such as steroids, for detection by MS/MS.4 Dansyl chloride is a fluorescent probe for proteins and enzymes.5 It displays excitation/emission maxima of 340/535 nm, respectively, in acetone.
1.Walker, J.M.The dansyl method for identifying N-terminal amino acidsMethods in molecular biology: Basic protein and peptide protocols32321-328(1994) 2.Takeuchi, T.HPLC of amino acids as dansyl and dabsyl derivativesJ. Chromatogr. Lib.70229-241(2005) 3.Hunter, K.J.A dansyl chloride-HPLC method for the determination of polyaminesMethods in molecular biology: Polyamine protocols79119-123(1998) 4.Santa, T.Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometryBiomed. Chromatogr.25(1-2)1-10(2011) 5.Chen, R.F., and Scott, C.H.Atlas of fluorescence spectra and lifetimes of dyes attached to proteinAnal. Lett.18(4)393-421(1985)