D-α-Tocopherol Succinate (Vitamin E succinate) is an antioxidant tocopherol and a salt form of vitamin E. D-α-Tocopherol Succinate inhibits Cisplatin -induced cytotoxicity. D-α-Tocopherol Succinate can be used for the research of cancer[1][2].
D-α-Tocopherol Succinate (1-20 μM; 24 hours) shows cytotoxicity to HEI-OC1 cells[1].
D-α-Tocopherol Succinate (10 μM; 48 hours) protects HEI-OC1 cells against cisplatin-induced ototoxicity and inhibits caspase-3 activity[1].
D-α-Tocopherol Succinate (0-50 μM; 18 hours) shows cytotoxicity to TC-1 tumor cells[2].
Cell Cytotoxicity Assay[1]
| Cell Line: | HEI-OC1 cell line |
| Concentration: | 1-20 μM |
| Incubation Time: | 24 hours |
| Result: | Significantly induced cytotoxicity at a concentration of 20 μM and showed a higher cytotoxicity potency compared with 10 μM. |
Cell Viability Assay[1]
| Cell Line: | HEI-OC1 cell line |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Increased cisplatin-induced cell population. Inhibited cisplatin-induced necrotic, ROS production and late apoptosis. Decreased cleaved PARP and inhibited the expression of caspase-3 which related to cisplatin-induced apoptosis. |
Cell Cytotoxicity Assay[2]
| Cell Line: | TC-1 tumor cells |
| Concentration: | 0, 25 and 50 μM |
| Incubation Time: | 18 hours |
| Result: | Dose-dependently showed cytotoxic and induced a higher percentage of necrotic TC-1 cells as opposed to apoptotic cells. |
D-α-Tocopherol Succinate (1-2 mg/kg; i.p. three times at 2 day intervals from TC-1 tumor cells injection for 10 days to 14 days) shows antitumor effects to mice with TC-1 tumor[2].
| Animal Model: | Six- to eight-week-old female C57BL/6 mice with TC-1 tumor cells[2] |
| Dosage: | 1 and 2 mg/kg |
| Administration: | Intraperitoneal injection; 1 and 2 mg/kg three times at 2 day intervals; from TC-1 tumor cells injection for 10 days to 14 days |
| Result: | Dreased the tumor volume, especially with a dose of 2 mg/kg. |
[1]. Kim SK, et al. The effects of the antioxidant α-tocopherol succinate on cisplatin-induced ototoxicity in HEI-OC1 auditory cells. Int J Pediatr Otorhinolaryngol. 2016 Jul;86:9-14.
[2]. Kang TH, et al. Treatment of tumors with vitamin E suppresses myeloid derived suppressor cells and enhances CD8+ T cell-mediated antitumor effects. PLoS One. 2014 Jul 29;9(7):e103562.