Cystathionine β-lyase, Recombinant Microorganisms (CBL) is an enzyme that catalyzes the breakdown of cystathionine to homocysteine, the penultimate step in methionine biosynthesis. Cystathionine β-lyase is important for bacterial virulence.
Cystathionine β-lyase (25 nM; 10 min) catalyzes L-(+)-cystathionine (100 μM) to homocysteine. Cystathionine β-lyase activity can be measured by monitoring the formation of homocysteine at 412 nm, using 5,5′-dithio-bis-(2-nitrobenzoic acid) as a detector of free thiol[1].
References:
[1]. Ejim LJ, et al. Cystathionine beta-lyase is important for virulence of Salmonella enterica serovar Typhimurium. Infect Immun. 2004 Jun;72(6):3310-4.