Cypridina luciferin is a chemiluminescent substrate for luciferase.1 Upon hydrolysis by luciferase, it displays a luminescence maximum of 465 nm. Cypridina luciferin has been used in estrogen receptor α (ERα) reporter assays for the discovery of ERα-activating compounds in vitro and in bioluminescence resonance energy transfer (BRET) assays for tumor detection in vivo.2,3
References:
[1].Shimomura, O., Johnson, F.H., and Masugi, T.Cypridina bioluminescence: Light-emitting oxyluciferin-luciferase complexScience164(3885)1299-1300(1969).
[2].Tochigi, Y., Sato, N., Sahara, T., et al.Sensitive and convenient yeast reporter assay for high-throughput analysis by using a secretory luciferase from Cypridina noctilucaAnal. Chem.82(13)5768-5876(2010).
[3].Wu, C., Mino, K., Akimoto, H., et al.In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dyeProc. Natl. Acad. Sci. USA106(37)15599-15603(2009).