Cyclo(Arg-Gly-Asp-D-Phe-Cys), commonly abbreviated as cRGDfC, is a cyclic RGD peptide that shows high affinity and selectivity for the integrin αvβ3 [1]. Treatment of murine embryonic stem cells (mESCs) with Cyclo(Arg-Gly-Asp-D-Phe-Cys) peptide can induce some cells to express early ectoderm lineage markers [2]. Cyclo(Arg-Gly-Asp-D-Phe-Cys) is mainly used to modify liposomes and other delivery systems to achieve targeted drug delivery to tumor tissues [3-4].
In murine embryonic stem cells, Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5mM; 24 h) down-regulated the transcription factors Oct 4, Sox 2, and Nanog [2].
References:
[1]. Nallamothu R, Wood GC, Pattillo CB, et al. A tumor vasculature targeted liposome delivery system for combretastatin A4: design, characterization, and in vitro evaluation. Aaps Pharmscitech. 2006 Jun; 7: E7-16.
[2]. Hazenbiller O, Duncan NA, Krawetz RJ. Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide. BMC Cell Biology. 2017 Dec; 18: 1-5.
[3]. Chen Z, Deng J, Zhao Y, et al. Cyclic RGD peptide-modified liposomal drug delivery system: enhanced cellular uptake in vitro and improved pharmacokinetics in rats. International journal of nanomedicine. 2012 Jul 18: 3803-3811.
[4]. Xia Y, Chen Y, Hua L, et al. Functionalized selenium nanoparticles for targeted delivery of doxorubicin to improve non-small-cell lung cancer therapy. International Journal of Nanomedicine. 2018 Oct 30: 6929-6939.
Cyclo(Arg-Gly-Asp-D-Phe-Cys)，通常缩写为cRGDfC，是一种环状RGD肽，对整合素αvβ3具有高亲和力和选择性 [1]。用Cyclo(Arg-Gly-Asp-D-Phe-Cys)肽处理小鼠胚胎干细胞（mESC）可诱导部分细胞表达早期外胚层谱系标志物 [2]。Cyclo(Arg-Gly-Asp-D-Phe-Cys)主要用于修饰脂质体和其他递送系统，以实现药物向肿瘤组织的靶向递送 [3-4]。
在小鼠胚胎干细胞中，Cyclo(Arg-Gly-Asp-D-Phe-Cys)（0.5mM；24h）下调了转录因子Oct 4、Sox 2和Nanog [2]。