Cyanine5 azide

Cyanine5 azide is a derivative of the fluorescent dye Cyanine5 and is commonly used in CuAAC click reactions ^[1]. Cyanine5 azide can react with mCherry-labeled tetravalent single-chain avidin (scAVD) proteins to form a tetrafluorophore nanoprobe, which allows the tracking of endoplasmic reticulum (ER) remodeling with nanometer spatial resolution ^[2]. Cyanine5 azide can be used to incubate cells to detect the possible DBCO groups expressed on the cell surface, and can be used to modify silica nanocomplexes to achieve efficient targeted delivery^[3]. Cyanine5 azide can be used to couple with the alkyne functional groups of phosphoramide morpholine oligonucleotides (PMO)-peptides to analyze the cellular uptake of peptide-PMO complexes in mouse brain endothelial cells (bEND5)^[4].

References:
[1] Huppelschoten Y, Elhebieshy A F, Hameed D S, et al. Total chemical synthesis of a functionalized GFP nanobody[J]. Chembiochem, 2022, 23(19): e202200304.
[2] Hou Y, Arai S, Kitaguchi T, et al. Intracellular bottom-up generation of targeted nanosensors for single-molecule imaging[J]. Nanoscale, 2016, 8(6): 3218-3225.
[3] Wang H, Tang L, Liu Y, et al. In vivo targeting of metabolically labeled cancers with ultra-small silica nanoconjugates[J]. Theranostics, 2016, 6(9): 1467.
[4] Shabanpoor F, Gait M J. Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry[J]. Chemical Communications, 2013, 49(87): 10260-10262.

Cyanine5 azide是荧光染料Cyanine5的衍生物，常用于CuAAC点击化学反应^[1]。Cyanine5 azide可与mCherry标记的四价单链亲和素蛋白(scAVD)反应，形成四荧光团纳米探针，能够以纳米级的空间分辨率追踪内质网（ER）的重塑过程^[2]。Cyanine5 azide可用于孵育细胞以检测细胞表面可能表达的DBCO基团，也可用于修饰二氧化硅纳米复合物以实现高效的靶向递送^[3]。Cyanine5 azide可用于与磷酰二胺吗啉代寡核苷酸(PMO)-肽的炔基官能团偶联，以分析肽-PMO复合物在小鼠脑内皮细胞(bEND5)中的细胞摄取情况^[4]。