Cyanine5-UTP (Cy5-UTP) can replace UTP as the substrate of T7 RNA polymerase, and generate orange fluorescently labeled mRNA through in vitro transcription, excitation/emission (nm): 650/665nm[1]. Probes generated using Cy5-UTP as a substrate are suitable for multicolor fluorescence analysis, especially dual-color expression arrays combined with Cy5-UTP [2].
References:
[1]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017 Jul;26(7):1363-1379.
[2]. Guerra (2006) Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques 41 (1):53.
Cyanine5-UTP (Cy5-UTP)可替代UTP作为T7 RNA聚合酶的底物,通过体外转录生成带有橙色荧光标记的mRNA,激发/发射(nm):650/665nm [1]。使用Cy5-UTP作为底物产生的探针适用于多色荧光分析,特别是与Cy5-UTP结合的双色表达阵列[2]。