Cy5 OVA mRNA (5'CAP) is produced through in vitro transcription, which simulates the mRNA processing process in eukaryotes. It has a 5 'end Cap 1 cap structure, a 3' end poly (A) tail, and Cy5-UTP modification (Cy5-UTP: UTP=3:1 (molar ratio)), increasing the stability and translation efficiency of mRNA[1]. Cy5 is a commonly used cyanine fluorescent dye with maximum excitation/emission wavelengths of 650/670nm, capable of real-time monitoring of the transfection, localization, and expression of target proteins in cells.
Ovalbumin (OVA) is a member of the chromoprotein superfamily and the main protein component in egg white. OVA is a glycoprotein with a molecular weight of approximately 45000 daltons that can induce moderate immunity in the body and is a commonly used antigen in immune and biochemical research. Egg white protein is often used as an immunogen for immune experiments, such as establishing animal models of high altitude sickness, asthma, etc. OVA mRNA can directly express proteins in the cytoplasm without relying on promoters, with a faster protein expression rate than transfected DNA. The protein expression level is directly related to the mRNA transfection level, and there is no risk of gene integration.
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122.
Cy5 OVA mRNA (5'CAP) 是通过体外转录产生的，通过模拟真核生物中mRNA加工过程具有5'端Cap 1帽结构、3'端poly(A)尾以及Cy5-UTP修饰（Cy5-UTP：UTP=3:1（摩尔比）），增加了mRNA的稳定性和翻译效率[1]。Cy5是一种常用的花青类荧光染料，最大激发/发射光波长分别为650/670nm，能够实时监测细胞中目的蛋白的转染、定位和表达情况。
卵白蛋白(Ovalbumin，OVA)是色蛋白超家族的成员，也是蛋清中的主要蛋白成分。OVA是一种分子量约45000道尔顿的糖蛋白，能够引起机体适度的免疫性，是免疫和生化研究的常用抗原。卵白蛋白常被用作免疫原进行免疫实验，如高原反应性、哮喘等动物模型建立。OVA mRNA能够在不依赖于启动子的情况下直接在细胞质中表达蛋白，蛋白表达速度比转染DNA更快，蛋白表达量与mRNA的转染量直接相关，并且没有基因整合的风险。