Cy5 mCherry mRNA (5'CAP) is produced through in vitro transcription. By simulating the mRNA processing process in eukaryotes, Cy5 mCherry mRNA (5'CAP) has a 5 'end Cap 1 cap structure, a 3' end poly (A) tail, and Cy5-UTP modification (Cy5-UTP: UTP=3:1 (molar ratio)), which increases the stability and translation efficiency of mRNA[1]. Cy5 is a commonly used cyanine fluorescent dye with maximum excitation/emission wavelengths of 650/670nm, capable of real-time monitoring of the transfection, localization, and expression of target proteins in cells.
Cy5 mCherry mRNA (5'CAP) can directly express proteins in the cytoplasm without relying on promoters, with a faster protein expression rate than transfected DNA. The protein expression level is directly related to the mRNA transfection level, and there is no risk of gene integration. After transfection of Cy5 mCherry mRNA (5'CAP) into cells, strong and bright red fluorescent protein mCherry can be expressed, with excitation/emission wavelengths of 587/610nm, respectively. Cy5 mCherry mRNA (5'CAP) can be used as a standard to detect the transfection efficiency of different transfection reagents, and can also be used as a control to study the transformation of fluorescent proteins in mammalian cells.
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122.
Cy5 mCherry mRNA (5'CAP)是通过体外转录产生的，通过模拟真核生物中mRNA加工过程具有5'端Cap 1帽结构、3'端poly(A)尾以及Cy5-UTP修饰（Cy5-UTP：UTP=3:1（摩尔比）），增加了mRNA的稳定性和翻译效率[1]。Cy5是一种常用的花青类荧光染料，最大激发/发射光波长分别为650/670nm，能够实时监测细胞中目的蛋白的转染、定位和表达情况。
Cy5 mCherry mRNA (5'CAP)能够在不依赖于启动子的情况下直接在细胞质中表达蛋白，蛋白表达速度比转染DNA更快，蛋白表达量与mRNA的转染量直接相关，并且没有基因整合的风险。Cy5 mCherry mRNA (5'CAP)转染细胞后能够表达强烈且明亮的红色荧光蛋白mCherry，激发/发射光波长分别为587/610nm。Cy5 mCherry mRNA (5'CAP)能够作为标准品检测不同转染试剂的转染效率，也能够作为对照研究哺乳动物细胞中荧光蛋白的转染和表达。