Cy5-DBCO is a bioorthogonal fluorescent probe that combines the near-infrared dye Cy5 with dibenzocyclooctyne (DBCO) (Ex=635nm, Em=650-700nm). Cy5-DBCO enables efficient and specific biomolecule labeling through a copper-free click chemistry reaction between the DBCO group and azides. Cy5-DBCO is used in applications such as bioimaging, protein labeling, cell tracing, and drug delivery system research[1-4].
References:
[1] Song S, Shim MK, Lim S, et al. In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjug Chem. 2020 May 20;31(5):1562-1574.
[2] Zhu L, Xu Y, Wei X, et al. Coupling Aptamer-based Protein Tagging with Metabolic Glycan Labeling for In Situ Visualization and Biological Function Study of Exosomal Protein-Specific Glycosylation. Angew Chem Int Ed Engl. 2021 Aug 9;60(33):18111-18115.
[3] Li L, Wang F, Mo S, et al. A Spatially Distributed Microneedle System for Bioorthogonal T Cell-Guided Cancer Therapy. Adv Sci (Weinh). 2025 Apr;12(13):e2416841.
[4] Xue Y, Li J, Chen D, et al. Observation of structural switch in nascent SAM-VI riboswitch during transcription at single-nucleotide and single-molecule resolution. Nat Commun. 2023 Apr 22;14(1):2320.
Cy5-DBCO是一种近红外荧光染料Cy5与二苯并环辛炔（DBCO）的生物正交荧光探针（Ex=635nm，Em=650-700nm）。Cy5-DBCO通过DBCO与叠氮化物的无铜点击化学反应实现高效、特异的生物分子标记。Cy5-DBCO可用于生物成像、蛋白质标记、细胞示踪以及药物递送系统的研究[1-4]。