Cy3-UTP (Cyanine 3-UTP) can replace UTP as the substrate of T7RNA polymerase to produce labeled probes through in vitro transcription. Λexc=550nm/Λem=570nm[1]. Cy3 dye gives the UTP molecule fluorescent properties, and Cy3-UTP can be incorporated into RNA molecules through the transcription reaction catalyzed by RNA polymerase. Enables researchers to track RNA distribution, localization and dynamics[2]. Cy3-UTP labeled mRNA is easily visible under UV light and does not require further staining after gel electrophoresis [3]. Cy3-UTP can be used for single-molecule fluorescence resonance energy transfer (FRET) measurement to monitor the co-transcriptional folding of RNA molecules in real time [4].This product is provided as a 10mM aqueous solution.
References:
[1]Bao W , Li A , Zhang Y ,et al. Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata[J].Plant Biotechnology Journal, 2021.
[2]Erika Schaudy,Jory Lietard, Mark M Somoza. Enzymatic Synthesis of High-Density RNA Microarrays[J]. Nucleic Acid Chemistry section. 2023 Feb;3(2):e667. doi: 10.1002/cpz1.667.
[3]Kim H, Park Y, Lee J B. Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression[J]. Scientific Reports, 2015, 5:12737.
[4]Uhm H , Kang W , Ha K S ,et al. Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch[J].Proceedings of the National Academy of Sciences, 2017, 115(2):201712983.
Cy3-UTP(Cyanine 3-UTP)可以取代UTP作为T7 RNA聚合酶的底物,通过体外转录产生标记探针。Λexc=550nm/Λem=570nm[1]。Cy3染料赋予UTP分子荧光特性,Cy3-UTP可以通过RNA聚合酶催化的转录反应掺入RNA分子中。使研究人员能够跟踪RNA的分布,定位和动态[2]。Cy3-UTP标记的mRNA在紫外光下容易可见,凝胶电泳后无需进一步染色[3]。Cy3-UTP可用于单分子荧光共振能量转移(FRET)测定,实时监测 RNA 分子的共转录折叠[4]。本产品以10mM水溶液形式提供。