Cy3-dUTP is a Cyanine3-labeled dUTP fluorescent dye. Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554nm, λem=568nm). Cy3-dUTP can serve as a substrate for T4 and Taq DNA polymerases, Escherichia coli DNA polymerases (whole enzymes and Klenow fragments), reverse transcriptases (from AMV and M-MuLV), and terminal transferases[1][2][3].
References:
[1] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Res. 1994 Aug 11;22(15):3226-32.
[2] Jiang Y, Li Z S, Jiang F S,et al. Effects of different ingredients of zedoary on gene expression of HSC-T6 cells. World J Gastroenterol. 2005 Nov 21;11(43):6780-6.
[3] Gutjahr A, Xu S Y. Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA. Nucleic Acids Res. 2014 May;42(9):e77.
Cy3-dUTP是一种cyanine3标记的dUTP荧光染料。Cy3 （Sulfo-Cyanine3）是蛋白质和核酸的橙色荧光标记（λex=554nm, λem=568nm）。Cy3-dUTP可以作为T4和Taq DNA聚合酶、大肠杆菌DNA聚合酶（全酶和Klenow片段）、逆转录酶（来自AMV和M-MuLV）和末端转移酶的底物[1][2][3]。