Coelenterazine h is a coelenterazine derivative and a bioluminescent molecule with a maximum excitation/emission wavelength of approximately 437/466nm[1, 2]. Coelenterazine h can serve as a highly efficient luminescent substrate for Renilla luciferase (Rluc) and its highly active mutant RLuc8[1]. Coelenterazine h exhibits high sensitivity to Ca2+, a property that makes it an excellent tool for measuring subtle changes in intracellular Ca2+ concentration[3].
References:
[1] Saito K, Hatsugai N, Horikawa K, et al. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level[J]. PLoS One, 2010, 5(4): e9935.
[2] Chemiluminescence and bioluminescence: past, present and future[M]. Royal Society of Chemistry, 2011.
[3] Krasitskaya V V, Bashmakova E E, Frank L A. Coelenterazine-dependent luciferases as a powerful analytical tool for research and biomedical applications[J]. International journal of molecular sciences, 2020, 21(20): 7465.
Coelenterazine h是一种腔肠素衍生物，是一种发光生物分子，最大激发/发射波长约为437/466nm[1, 2]。Coelenterazine h能够作为RLuc8的高效发光底物[1]。Coelenterazine h对Ca2+具有高度的敏感性，这一特性使其成为测量细胞内Ca2+浓度微小变化的优异工具[3]。