Clonostachydiol is a fungal metabolite originally isolated from Clonostachys cylindrospora that has anticancer and anthelmintic activities.1,2 It is cytotoxic to P388 (IC50 = 25 µM), as well as L1210, HT-29, and A549 cancer cells (IC50s = 4.5, 4.2, and 5.7 µg/ml, respectively). Clonostachydiol (2.5 mg/kg, s.c.) reduces fecal worm burden by 80 to 90% in lambs infected with the nematode H. contortus.1
1.Grabley, S., Hammann, P., Thiericke, R., et al.Secondary metabolites by chemical screening. 21. Clonostachydiol, a novel anthelmintic macrodiolide from the fungus Clonostachys cylindrospora (strain FH-A 6607)J. Antibiot. (Tokyo)46(2)343-345(1993) 2.Lang, G., Mitova, M.I., Ellis, G., et al.Bioactivity profiling using HPLC/microtiter-plate analysis: Application to a New Zealand marine alga-derived fungus, Gliocladium spJ. Nat. Prod.69(4)621-624(2006)