Clerosterol is a phytosterol that has been found in C. fragile and has diverse biological activities.[1],[2] It is active against T. b. brucei (IC50 = 53.6 µg/ml).[1] Clerosterol inhibits the proliferation of A2058 melanoma cells (IC50 = 150 µM).[2] It induces apoptosis, cell cycle arrest at the sub-G1 phase, and mitochondrial membrane depolarization in A2058 cells when used at a concentration of 150 µM.
References:
[1].Hoet, S., Pieters, L., Muccioli, G.G., et al.Antitrypanosomal activity of triterpenoids and sterols from the leaves of Strychnos spinosa and related compoundsJ. Nat. Prod.70(8)1360-1363(2007).
[2].Kim, A.D., Lee, Y., Kang, S.-H., et al.Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cellsMar. Drugs11(2)418-430(2013).