Chrysin is a natural flavonoid found in various plant extracts, honey, and propolis, possessing diverse biological activities, including anti-inflammatory, antioxidant, and anticancer effects[1, 2]. Chrysin can regulate drug resistance by inhibiting P-glycoprotein activity, thereby increasing the intracellular concentration of chemotherapeutic drugs such as paclitaxel[3].
In vitro, treatment of DU145 and PC-3 cells with Chrysin (0-100µM) for 48h induced apoptosis, evidenced by DNA fragmentation and an increase in the number of cells in the sub-G1 phase of the cell cycle in both DU145 and PC-3 cells[4]. Treatment of U937 cells with Chrysin (5-10µM) for 12h induced apoptosis and significantly induced the release of cytochrome c from mitochondria into the cytoplasm[5]. Treatment of human melanoma cells (SP6.5 and M17 cells) with Chrysin (0-100µM) for 12h reduced cell viability in a dose-dependent manner, with IC50 values of 28.3µM and 35.8µM for SP6.5 and M17 cells, respectively[6].
In vivo, oral administration of Chrysin (5, 10mg/kg) to mice with colitis for 7 days significantly improved the damage to the colonic tissue structure, reduced colonic myeloperoxidase (MPO) activity, and decreased the production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines[7].
References:
[1] Zeinali M, Rezaee S A, Hosseinzadeh H. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances[J]. Biomedicine & Pharmacotherapy, 2017, 92: 998-1009.
[2] Stompor-Gorący M, Bajek-Bil A, Machaczka M. Chrysin: Perspectives on contemporary status and future possibilities as pro-health agent[J]. Nutrients, 2021, 13(6): 2038.
[3] Panneerselvam S, Hussain S, Jeyavelkumaran R. Chrysin as a Chemosensitizer: Molecular Insights into Its Role in Prostate Cancer Treatment[J]. Journal of Bio-X Research, 2025, 2025: 0060.
[4] Ryu S, Lim W, Bazer F W, et al. Chrysin induces death of prostate cancer cells by inducing ROS and ER stress[J]. Journal of cellular physiology, 2017, 232(12): 3786-3797.
[5] Woo K J, Jeong Y J, Park J W, et al. Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells[J]. Biochemical and biophysical research communications, 2004, 325(4): 1215-1222.
[6] Xue C, Chen Y, Hu D N, et al. Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis[J]. Oncology Letters, 2016, 12(6): 4813-4820.
[7] Shin E K, Kwon H S, Kim Y H, et al. Chrysin, a natural flavone, improves murine inflammatory bowel diseases[J]. Biochemical and biophysical research communications, 2009, 381(4): 502-507.
Chrysin是一种天然的黄酮，存在于多种植物提取物、蜂蜜和蜂胶中，具有多种生物活性，包括抗炎、抗氧化、抗癌等作用[1, 2]。Chrysin能够通过抑制P糖蛋白活性调节药物耐药性，从而提高如紫杉醇等化疗药物的细胞内浓度[3]。
在体外，Chrysin（0-100µM）处理DU145和PC-3细胞48h，诱导了细胞凋亡，表现为DNA片段化，并增加了DU145和PC-3细胞在细胞周期的亚G1期细胞数量[4]。Chrysin（5-10µM）处理U937细胞12h，诱导了细胞凋亡，显著诱导细胞色素c从线粒体释放到细胞质中[5]。Chrysin（0-100µM）处理人黑色素瘤细胞（SP6.5和M17细胞）12h，以剂量依赖性方式降低了细胞的活力，对SP6.5和M17细胞的IC50值分别为28.3µM和35.8µM[6]。
在体内，Chrysin（5, 10mg/kg）通过口服治疗结肠炎小鼠7天，显著改善了小鼠的结肠组织结构的破坏，降低了结肠髓过氧化物酶（MPO）活性，减少了一氧化氮（NO）、前列腺素E2（PGE2）和促炎细胞因子等炎症介质的产生[7]。