Chlorophenol Red β-D-Galactopyranoside is a long-wavelength dye, which can be detected with an absorbance at 570nm[1]. Chlorophenol Red β-D-Galactopyranoside has been widely used to detect β-galactosidase in bacteria and plants [2-3]. The anti-HIV activity of MAGI cells can be clearly detected by Chlorophenol Red β-D-Galactopyranoside with high sensitivity using standard spectrometers[4]. Chlorophenol Red β-D-Galactopyranoside showed high accuracy, allowing detection of enzyme activity at concentrations as low as 0.05mU, independent of blood contamination[5].
References:
[1] Shin H J, Park H H, Lim W K. Freeze-dried recombinant bacteria for on-site detection of phenolic compounds by color change[J]. Journal of biotechnology, 2005, 119(1): 36-43.
[2] Sicard C, Shek N, White D, et al. A rapid and sensitive fluorimetric β-galactosidase assay for coliform detection using chlorophenol red-β-D-galactopyranoside[J]. Analytical and bioanalytical chemistry, 2014, 406(22): 5395-5403.
[3] Micieta K, Tokhtaeva E, Stano J, et al. A simple and rapid method of identification of extracellular plant galactosidases[J]. Chemistry of natural compounds, 2002, 38(3): 284-287.
[4] Miyamoto F, Kawaji K, Oishi S, et al. Anti-HIV-1 activity determined by β-galactosidase activity in the multinuclear activation of an indicator assay is comparable with that by a conventional focus counting method[J]. Antiviral Chemistry and Chemotherapy, 2015, 24(2): 77-82.
[5] Pelisek J, Armeanu S, Nikol S. Evaluation of β-galactosidase activity in tissue in the presence of blood[J]. Journal of vascular research, 2000, 37(6): 585-593.
Chlorophenol Red β-D-Galactopyranoside是一种长波长染料，可在570nm波长处检测吸光度[1]。Chlorophenol Red β-D-Galactopyranoside广泛应用于细菌和植物中β-半乳糖苷酶的检测[2-3]。使用标准光谱仪时，应用Chlorophenol Red β-D-Galactopyranoside能以高灵敏度清晰检测MAGI细胞的抗HIV活性[4]。Chlorophenol Red β-D-Galactopyranoside具有高精确度，可检测低至0.05mU的酶活性，且不受血液污染的影响[5]。