Chlorin e6(Ce6) is a second-generation photosensitizer. Compared to the first-generation photosensitizers, Chlorin e6 has the advantage of efficiently absorbing longer light wavelengths, which is favorable for deeper tissue penetration[1] .Chlorin e6-based photosensitizers are widely used in antitumor photodynamic therapy(PDT) due to high quantum output of single oxygen and a strong absorption band in a red region[2]
Chlorin e6-mediated PDT inhibits adipocyte differentiation and lipogenesis via regulating AMPK in 3T3-L1 cells, indicated that Chlorin e6-mediated PDT might serve as a potential therapy for the treatment of obesity and obesity-associated diseases[2]. Chlorin e6-Fu/AL@GG hydrogel can be a feasible nanocarrier for Chlorin e6-assisted PDT that possesses an excellent capability to selectively kill colon cancer cells[3]
Chlorin e6-loaded PEG-PCL nanoemulsions (Ce6-PCL-NEs) showed efficient cellular uptake and, upon laser irradiation, generated singlet oxygen to kill tumor cells. Particularly, Chlorin e6-PCL-NEs showed prolonged blood circulation and about 60% increased tumor accumulation compared to free Chlorin e6 after intravenous injection to 4T1 tumor-bearing mice(2.5mg/kg), indicated the promising potential of Chlorin e6-PCL-NEs for efficient PDT and in vivo drug delivery to tumor tissue[4]. Chlorin e6 nano-precipitations (Chlorin e6 NPs) can be prepared by a one-pot method for effective photodynamic therapy of colorectal cancer. The HT-29 tumour-bearing mice were randomly divided into three groups and were administered intravenously with saline, free Chlorin e6 and Chlorin e6 NPs (5mg/kg Chlorin e6) once every 2 days for 2 weeks. The laser was applied three times 24h post injection (680 nm, 0.5 W/cm2 for 5 min). Chlorin e6 NPs showed significantly enhanced anticancer benefits compared to free Chlorin e6, which almost obtained full ablation of tumours at the end of the study[5]
References:
[1].Ryu AR, Kim YW, et al. Chlorin e6-mediated photodynamic therapy modulates adipocyte differentiation and lipogenesis in 3T3-L1 cells. Photodiagnosis Photodyn Ther. 2020;31:101917.
[2].Papayan G.V., Akopov А.L. Photodynamic theranostics of central lung cancer: capabilities of early diagnosis and minimally invasive therapy (review). Sovremennye tehnologii v medicine 2021; 13(6): 78
[3].Karuppusamy S, Hyejin K, et al. Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer. Colloids Surf B Biointerfaces. 2019;181:778-788.
[4].Park C, Yoo J, et al. Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery. Int J Mol Sci. 2019;20(16):3958. Published 2019 Aug 14.
[5].Miao Z, Wang Y, et al. One-pot synthesis chlorin e6 nano-precipitation for colorectal cancer treatment Ce6 NPs for colorectal cancer treatment. IET Nanobiotechnol. 2021;15(8):680-685.
Chlorin e6(Ce6) 是第二代光敏剂。与第一代光敏剂相比，Chlorin e6具有高效吸收更长波长光的优势，有利于更深的组织穿透[1]。基于Chlorin e6的光敏剂广泛应用于抗肿瘤光动力治疗(PDT) 由于单氧的高量子输出和红色区域的强吸收带[2]
Chlorin e6 介导的 PDT 通过调节 3T3-L1 细胞中的 AMPK 抑制脂肪细胞分化和脂肪生成，表明 Chlorin e6 介导的 PDT 可能作为治疗肥胖和肥胖相关疾病的潜在疗法[2 ]。 Chlorin e6-Fu/AL@GG 水凝胶可作为 Chlorin e6 辅助 PDT 的纳米载体，具有出色的选择性杀死结肠癌细胞的能力[3]
负载二氢卟酚 e6 的 PEG-PCL 纳米乳剂 (Ce6-PCL-NEs) 显示出有效的细胞摄取，并且在激光照射下产生单线态氧以杀死肿瘤细胞。特别是，在静脉注射给 4T1 荷瘤小鼠（2.5mg/kg）后，与游离的 Chlorin e6 相比，Chlorin e6-PCL-NEs 显示出延长的血液循环和约 60% 的肿瘤积累，表明 Chlorin e6-PCL-用于有效 PDT 和体内药物递送至肿瘤组织的 NEs[4]。可通过一锅法制备二氢卟酚 e6 纳米沉淀物 (Chlorin e6 NPs)，用于结直肠癌的有效光动力治疗。 HT-29 荷瘤小鼠随机分为三组，每 2 天一次静脉注射生理盐水、游离 Chlorin e6 和 Chlorin e6 NPs (5mg/kg Chlorin e6)，持续 2 周。注射后 24 小时应用激光 3 次（680 nm，0.5 W/cm2，持续 5 分钟）。与游离的 Chlorin e6 相比，Chlorin e6 NPs 显示出显着增强的抗癌益处，后者在研究结束时几乎完全消融了肿瘤[5]