Chlorambucil-d8 is intended for use as an internal standard for the quantification of chlorambucil . Chlorambucil is a nitrogen mustard alkylating agent that forms intra- and interstrand crosslinks in DNA, the latter of which prevents DNA replication and transcription leading to cell death.1 It preferentially decreases survival of rapidly proliferating spleen colony-forming cells over spleen colony-forming cells from normal bone marrow.2 Formulations containing chlorambucil have been used alone and in combination with other chemotherapeutic agents in the treatment of chronic lymphocytic leukemia and lymphomas.
1.Mattes, W.B., Hartley, J.A., and Kohn, K.W.DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustardsNucleic Acids Res.14(7)2971-2987(1986) 2.van Putten, L.M., and Lelieveld, P.Factors determining cell killing by chemotherapeutic agents in vivo. II. Melphalan, chlorambucil and nitrogen mustardEur. J. Cancer7(1)11-16(1971)