Chicoric acid是一种羟基肉桂酸，属于苯丙醇类有机化合物，存在于菊苣、 蒲公英和罗勒等多种植物中，具有抗氧化、抗炎、抗糖尿病、抗病毒等多种生物活性。
Cas No.:6537-80-0
Sample solution is provided at 25 µL, 10mM.
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Chicoric acid is a hydroxycinnamic acid, belonging to the phenylpropanoid class of organic compounds. Chicoric acid is found in various plants, including chicory, dandelion, and basil, and possesses multiple biological activities such as antioxidant, anti-inflammatory, anti-diabetic, and antiviral effects[1, 2]. Chicoric acid can promote insulin release and glucose uptake[3]. Chicoric acid can alleviate cognitive impairment caused by oxidative stress[4].
In vitro, pretreatment of human umbilical vein endothelial cells (HUVECs) with Chicoric acid (25-200µM) for 30min inhibited apoptosis induced by high glucose/high fat (HG+HF) medium, and reduced HG+HF-induced IκBα phosphorylation and degradation, as well as subsequent p65 NFκB nuclear translocation[5]. Treatment of 3T3-L1 preadipocytes with Chicoric acid (10-200µM) for 24-72h inhibited cell viability in a dose- and time-dependent manner, induced apoptosis, and reduced mitochondrial membrane potential (MMP)[6].
In vivo, Chicoric acid (20mg/kg) administered via intraperitoneal injection to mice with compound 48/80-induced anaphylactic shock reduced serum histamine levels and mortality[7].
References:
[1] Duda Ł, Kłosiński K K, Budryn G, et al. Medicinal use of chicory (Cichorium intybus L.)[J]. Scientia Pharmaceutica, 2024, 92(2): 31.
[2] Ferrare K, Bidel L P R, Awwad A, et al. Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory (Cichorium intybus L.) for an antidiabetic effect[J]. Journal of ethnopharmacology, 2018, 215: 241-248.
[3] Tousch D, Lajoix A D, Hosy E, et al. Chicoric acid, a new compound able to enhance insulin release and glucose uptake[J]. Biochemical and biophysical research communications, 2008, 377(1): 131-135.
[4] Wang Y, Diao Z, Li J, et al. Chicoric acid supplementation ameliorates cognitive impairment induced by oxidative stress via promotion of antioxidant defense system[J]. RSC advances, 2017, 7(57): 36149-36162.
[5] Ma X, Zhang J, Wu Z, et al. Chicoric acid attenuates hyperglycemia-induced endothelial dysfunction through AMPK-dependent inhibition of oxidative/nitrative stresses[J]. Journal of Receptors and Signal Transduction, 2021, 41(4): 378-392.
[6] Xiao H, Wang J, Yuan L, et al. Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways[J]. Journal of agricultural and food chemistry, 2013, 61(7): 1509-1520.
[7] Lee N Y, Chung K S, Jin J S, et al. Effect of chicoric acid on mast cell-mediated allergic inflammation in vitro and in vivo[J]. Journal of natural products, 2015, 78(12): 2956-2962.
Chicoric acid是一种羟基肉桂酸，属于苯丙醇类有机化合物，存在于菊苣、 蒲公英和罗勒等多种植物中，具有抗氧化、抗炎、抗糖尿病、抗病毒等多种生物活性[1, 2]。Chicoric acid能够促进胰岛素释放和葡萄糖吸收[3]。Chicoric acid能够缓解由氧化应激引起的认知障碍[4]。
在体外，Chicoric acid（25-200µM）预处理人脐静脉内皮细胞（HUVECs）30min，抑制了高糖/高脂（HG+HF）培养基诱导的HUVECs凋亡，减少了HG+HF诱导的IκBα磷酸化和降解，以及随后的p65 NFκB核转位[5]。Chicoric acid（10-200µM）处理3T3-L1前脂肪细胞24-72h，以剂量和时间依赖性方式抑制了细胞活力，诱导了细胞凋亡，降低了线粒体膜电位（MMP）[6]。
在体内，Chicoric acid（20mg/kg）通过腹腔注射治疗化合物48/80诱发的过敏性休克小鼠，降低了小鼠血清组胺水平和死亡率[7]。
| Cell experiment [1]: | |
Cell lines | Human umbilical vein endothelial cells (HUVECs) |
Preparation Method | Cells were pretreated with different doses of chicoric acid (25, 50, 100, 200µM) for 30min before exposure to high glucose/high fat (HGþHF) medium supplemented with glucose (25mM) and saturated free fatty acid palmitate (500µM) for 24h. The cells in the control group were incubated with an equal amount of palmitate-free BSA and glucose (5mM). The apoptosis of HUVECs was determined by terminal deoxynucleotidy1 transferase-mediated dUTP nick end-labeling (TUNEL) assay. |
Reaction Conditions | 25, 50, 100, 200µM; 30min |
Applications | Chicoric acid inhibited HG1HF-induced apoptosis in HUVECs. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | ICR mice were injected intraperitoneally with PBS or compound 48/80 (8mg/kg dissolved in PBS). Chicoric acid at doses of 20mg/kg, 1h before compound 48/80 was injected. Mortality was monitored for 1h after induction of anaphylactic shock, after which blood was collected from the heart of each rat to measure serum histamine content. The blood was clotted for 1h at room temperature and centrifuged for 20min at 3,000g at 4°C to obtain the serum. |
Dosage form | 20mg/kg; i.p. |
Applications | Chicoric acid at a dose of 20mg/kg decreased the compound 48/80-induced serum histamine levels. The results of the mortality test correlated with the measured serum histamine levels. |
References: | |
| Cas No. | 6537-80-0 | SDF | Download SDF |
| 别名 | 菊苣酸; Cichoric acid; Dicaffeoyltartaric acid | ||
| 分子式 | C22H18O12 | 分子量 | 474.37 |
| 溶解度 | Water : 100 mg/mL (210.81 mM; Need ultrasonic)|DMSO : 1 mg/mL (2.11 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1081 mL | 10.5403 mL | 21.0806 mL |
| 5 mM | 421.6 μL | 2.1081 mL | 4.2161 mL |
| 10 mM | 210.8 μL | 1.054 mL | 2.1081 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
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