Cetrimonium Bromide (CTAB), a common used surfactant, act as a drug solubilizer with intrinsic antibacterial properties [1]. Cetrimonium Bromide can reduce the germination rate of Brassica campestris, affect the root elongation after seed germination, decrease the chlorophyll content in plant leaves, and increase the antioxidant enzyme activity of plants[2]. Cetrimonium Bromide has been widely used for the rapid extraction of nucleic acids from plant tissues [3].
In vitro, Cetrimonium Bromide treatment for 48 hours significantly inhibited the viability of HOS, MG63 and U2OS cells, with IC50 values of 4.949, 3.500 and 4.212µM respectively[4]. 5.0μM Cetrimonium Bromide treatment for 16 hours significantly reduced the adhesion of SCC4 cells and altered cell morphology[5]. Treatment with 10µM Cetrimonium Bromide for 48 hours resulted in an increase in the number of apoptotic cells in NDRG1-deficient DU-145 cells, accompanied by an increase in cleaved PARP levels[6].
In vivo, Cetrimonium Bromide treatment (5mg/kg/day; i.p.) for 5 consecutive days can reduce the tumor-forming ability of FaDu cell-bearing mice and slow down tumor growth[7]. Cetrimonium Bromide (13.7mmol/kg) was injected via tail vein once every three days for 30 days, which significantly reduced the metastasis of breast cancer from the 4T1 orthotopic breast cancer model in mice to the lungs[8].
References:
[1] Carvalho G C, Marena G D, Karnopp J C F, et al. Cetyltrimethylammonium bromide in the synthesis of mesoporous silica nanoparticles: General aspects and in vitro toxicity[J]. Advances in Colloid and Interface Science, 2022, 307: 102746.
[2] Song U, Kim H E. Assessing the phytotoxicity of cetrimonium bromide in plants using eco-physiological parameters[J]. Journal of Ecology and Environment, 2016, 40(1): 14.
[3] Allen G C, Flores-Vergara M A, Krasynanski S, et al. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide[J]. Nature protocols, 2006, 1(5): 2320-2325.
[4] Da W, Tao L, Zhu Y. The inhibitory effect of CTAB on human osteosarcoma through the PI3K/AKT signaling pathway[J]. International Journal of Oncology, 2021, 59(1): 42.
[5] Yue C H, Chen C H, Pan Y R, et al. Cetyltrimethylammonium bromide disrupts mesenchymal characteristics of human tongue squamous cell carcinoma SCC4 cells through modulating canonical TGF-β/Smad/miR-181b/TIMP3 signaling pathway[J]. Anticancer Research, 2021, 41(12): 6095-6104.
[6] Wissing M D, Mendonca J, Kim E, et al. Identification of cetrimonium bromide and irinotecan as compounds with synthetic lethality against NDRG1 deficient prostate cancer cells[J]. Cancer Biology & Therapy, 2013, 14(5): 401-410.
[7] Ito E, Yip K W, Katz D, et al. Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer[J]. Molecular pharmacology, 2009, 76(5): 969-983.
[8] Li N, Chen Y, Yang Y, et al. Cetyltrimethylammonium bromide inhibits the metastasis of breast cancer to the lungs by inhibiting epithelial–mesenchymal transition[J]. Biocell, 2022, 46(6): 1473.
Cetrimonium Bromide (CTAB)是一种常用的表面活性剂，具有内在的抗菌特性，能够作为药物的增溶剂 [1]。Cetrimonium Bromide能降低Brassica campestris种子的萌发率，影响种子萌发后的根伸长，减少植物叶片中的叶绿素含量，并提高植物的抗氧化酶活性[2]。Cetrimonium Bromide已广泛用于从植物组织中快速提取核酸[3]。
在体外，Cetrimonium Bromide处理48小时显著抑制了HOS、MG63和U2OS细胞的活力，IC50值分别为4.949、3.500和4.212µM[4]。5.0µM的Cetrimonium Bromide处理16小时显著降低了SCC4细胞的粘附能力并改变了细胞形态[5]。10µM的Cetrimonium Bromide处理48小时导致NDRG1缺陷型DU-145细胞凋亡数量增加，并伴有裂解PARP水平的升高
在体内，连续5天腹腔注射Cetrimonium Bromide（5mg/kg/day）能够降低FaDu细胞-荷瘤小鼠的成瘤能力并减缓肿瘤生长[7]。每隔三天通过尾静脉注射Cetrimonium Bromide（13.7mmol/kg），持续30天，显著减少了4T1小鼠原位乳腺癌模型中的乳腺癌向肺部的转移[8]。