Cas9 mRNA (5'CAP) is produced through in vitro transcription. By simulating the mRNA processing process in eukaryotes, it has a 5' end Cap 1 cap structure and a 3' end poly(A) tail, which increases the stability and translation efficiency of the mRNA[1].
Bacteria and archaea use the CRISPR/Cas system to defend against invading viruses and plasmids. The CRISPR/Cas system works in eukaryotic systems through two molecular components: Cas9 protein and non-coding guide RNA (gRNA). Cas9 endonuclease reaches the target site under the guidance of gRNA and cuts the sequence, resulting in gene silencing.
This product should be used in conjunction with purified Guide RNA.
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122.
Cas9 mRNA (5'CAP) 是通过体外转录产生的,通过模拟真核生物中mRNA加工过程具有5'端Cap 1帽结构和3'端poly(A)尾,增加了mRNA的稳定性和翻译效率[1]。
细菌和古菌使用CRISPR / Cas系统来防御入侵的病毒和质粒。CRISPR/Cas系统通过两种分子成分在真核系统中发挥作用:Cas9 蛋白和非编码向导RNA(Guide RNA,gRNA)。Cas9核酸内切酶在gRNA的导向下到达目的位点并切割序列,从而导致基因沉默。
本产品应与纯化的Guide RNA结合使用。