C18 Ceramide-d7 (d18:1-d7/18:0) is a deuterated (d7) labeled C18 Ceramide isotope internal standard. Ceramide is a type of sphingolipid core molecule composed of a sphingosine (often d18:1) linked to a fatty acid via an amide bond[1]. Ceramides have been implicated in mediating or regulating numerous central cellular processes such as proliferation, differentiation and senescence, stress responses, apoptosis, and inflammation[2]. Harmful stimuli can activate ceramides, leading to a variety of pathological conditions[2]. C18 Ceramide is one type of ceramide containing 18-carbon fatty acids and is closely related to cardiovascular diseases[3]. C18 Ceramide-d7 is often used as an internal standard for LC-MS/MS to quantify C18 ceramide in plasma/serum/tissue/cells.
References:
[1] Kauhanen D, Sysi-Aho M, Koistinen KM, Laaksonen R, Sinisalo J, Ekroos K. Development and validation of a high-throughput LC-MS/MS assay for routine measurement of molecular ceramides. Anal Bioanal Chem. 2016;408(13):3475-3483.
[2] Maceyka M, Spiegel S. Sphingolipid metabolites in inflammatory disease. Nature. 2014;510(7503):58-67.
[3] Tarasov K, Ekroos K, Suoniemi M, Kauhanen D, Sylvänne T, Hurme R, et al. Molecular lipids identify cardiovascular risk and are efficiently lowered by simvastatin and PCSK9 deficiency. J Clin Endocrinol Metab. 2014;99:E45–52.
C18 Ceramide-d7 (d18:1-d7/18:0)是一种氘化（d7）标记的C18神经酰胺同位素内标。Ceramide是一类鞘脂（sphingolipid）核心分子，由一个鞘氨醇（sphingosine，常为d18:1）通过酰胺键连接一个脂肪酸（fatty acid）组成[1]。Ceramide参与介导或调节许多中枢细胞过程，如增殖、分化和衰老、应激反应、细胞凋亡和炎症[2]。有害刺激可激活神经酰胺，导致多种病理状况[2]。C18 Ceramide是一类含有18碳脂肪酸的神经酰胺，与心血管疾病密切相关[3]。C18 Ceramide-d7常作为LC-MS/MS定量血浆/血清/组织/细胞中C18神经酰胺的内标。