C12E8 is a non-ionic surfactant, its full name is surfactant polyoxyethylene glycol, it belongs to the polyoxyethylene ether surfactant [1]. C12E8 regulates the curvature and morphological transition of phospholipid vesicle membrane in a concentration-dependent manner, promoting natural morphological changes at low concentrations and inducing abnormal deformation and ultimately leading to vesicle disintegration at high concentrations [2-3].
In DC3F cells, C12E8 (0.05mg/mL; 45min) decreases the voltage required for irreversible electroporation in planar lipid bilayers [4]. In erythrocytes, large flat invaginations/endovesicles with a compressed central region and a globular periphery were found in sections of erythrocytes treated with C12E8 (44μM; 60 min) [5].
References:
[1]. Ruiz CC, Molina-Bolívar JA. Characterization of mixed non-ionic surfactants n-octyl-β-d-thioglucoside and octaethylene–glycol monododecyl ether: Micellization and microstructure. Journal of colloid and interface science. 2011 Sep 1; 361(1): 178-185.
[2]. Mavcic B, Babnik B, Iglic A, et al. Shape transformation of giant phospholipid vesicles at high concentrations of C12E8. Bioelectrochemistry. 2004 Jun 1; 63(1-2): 183-187.
[3]. Guo Y. Surface interactions between poly (ethylene glycol) and phospholipid bilayers: Effects on aggregation and fusion. State University of New York at Buffalo; 1993.
[4]. Kanduser M, Fosnaric M, Sentjurc M, et al. Effect of surfactant polyoxyethylene glycol (C12E8) on electroporation of cell line DC3F. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2003 Mar 12; 214(1-3): 205-217.
[5]. Bobrowska-Hägerstrand M, Kralj-Iglič V, Iglič A, et al. Torocyte membrane endovesicles induced by octaethyleneglycol dodecylether in human erythrocytes. Biophysical journal. 1999 Dec 1; 77(6): 3356-3362.
C12E8是一种非离子表面活性剂，全名为表面活性剂聚氧乙二醇，属于聚氧乙烯醚类表面活性剂 [1]。C12E8以浓度依赖性方式调节磷脂囊泡膜的曲率和形态转变，低浓度时促进囊泡膜的自然形态变化，高浓度时诱导囊泡膜异常变形，最终导致囊泡崩解 [2-3]。
在DC3F细胞中，C12E8（0.05 mg/mL；45分钟）降低了平面脂质双层膜中不可逆电穿孔所需的电压 [4]。在红细胞中，用C12E8（44μM；60分钟）处理的红细胞切片发现大的扁平内陷/内囊泡，具有压缩的中心区域和球状外围 [5]。