Broussochalcone A

Broussochalcone A is an antioxidant and an inhibitor of Xanthine Oxidase (IC50=2.21 μM), with free radical scavenging activity. Broussochalcone A inhibits iron-induced lipid peroxidation and nitric oxide synthesis in lipopolysaccharide (LPS) -activated macrophages. Broussochalcone A also induces Apoptosis of human renal carcinoma cells by increasing ROS levels and activating FOXO3 signaling pathways.

Broussochalcone A (0.3, 1, and 3 μM; 10 min, Fe induction for another 30 min) inhibits Fe²⁺ (200 μM)-induced lipid peroxidation in rat brain homogenate^[1].
Broussochalcone A (1-30 μM; 30 min) increases DPPH (100 μM)-scavenging activity dose-dependently^[1].
Broussochalcone A (0.1-1 μM) inhibits cytochrome c reduction with an IC50 value of 0.5 μM, mostly due to its superoxide anion-scavenging activity and only partially to its inhibition of xanthine oxidase activity^[1].
Broussochalcone A (1-20 μM; 24 h) inhibits nitrite production and iNOS protein expression^[1].

References:
[1]. Cheng Z, et al. Broussochalcone A, a potent antioxidant and effective suppressor of inducible nitric oxide synthase in lipopolysaccharide-activated macrophages. Biochem Pharmacol. 2001 Apr 15;61(8):939-46.
[2]. Lee HK, et al. Broussochalcone A Induces Apoptosis in Human Renal Cancer Cells via ROS Level Elevation and Activation of FOXO3 Signaling Pathway. Oxid Med Cell Longev. 2021 Oct 27;2021:2800706.