Bradford Protein Quantification Kit is a protein concentration determination kit with fast detection speed, good stability, high sensitivity, and the detection data has a good linear relationship.
This kit is developed based on the Bradford method, one of the two most commonly used protein concentration detection methods. It is one of the most sensitive protein concentration detection methods currently commonly used. After the Coomassie Brilliant Blue G-250 dye in the dye solution binds to the protein, the maximum absorption peak of the dye changes from A456 to A595, and the measured absorbance value is proportional to the protein concentration. Therefore, the protein concentration in the solution can be determined by detecting the absorbance at 595 nm, achieving a quick and easy protein concentration determination.
The detection speed of this kit is extremely fast. It takes less than 10 minutes to process 10-20 samples. Compared with the BCA method, the detection time is greatly shortened. It is not affected by the chemical reagents in most samples, and the detection results are reliable.
Bradford Protein Quantification Kit是蛋白浓度测定试剂盒，检测速度快，稳定性好，灵敏度高，并且检测数据有很好的线性关系。
本试剂盒是基于最常用的两种蛋白浓度检测方法之一Bradford法研制而成，是目前常用的灵敏度较高的蛋白浓度测定方法之一。染液中的考马斯亮蓝G-250染料与蛋白结合后，使染料的最大吸收峰从A456变为A595，且测定的吸光值与蛋白浓度成正比关系。因此，可通过检测595nm处的吸光度对溶液中蛋白质浓度进行测定，实现了快速简便的蛋白浓度测定。
本试剂盒检测速度极快，10-20个样品耗时不足10分钟，和BCA法相比大大缩短了检测时间，且不受大多数样品中化学试剂的影响，检测结果可靠。