BODIPY 665/676 is a lipophilic fluorescent probe with high affinity for binding free fatty acids and triglycerides, with a maximum absorption wavelength of long wavelength（ λ Ex=665nm) and maximum fluorescence emission wavelength (Em=676nm) [1]. Similar to the commonly used lipid peroxidation probe C11 BODIPY 581/591 (MX5211-1MG), BODIPY 665/676 is easily oxidized by peroxides. Once oxidized, the maximum emission wavelength shifts to 605nm（ λ Ex=580 nm) [2-4]. The most important advantage of BODIPY 665/676 is that its emission wavelength is 676nm, which does not cross with the majority of fluorescein, thus avoiding interference such as self fluorescence. BODIPY 665/676 exhibits thermal stability and high hydrophobicity, thus preventing the diffusion of lipid droplets. The exciter at 642nm is close to the maximum absorption wavelength at 665nm, thus obtaining a strong fluorescence signal at very low probe concentrations [5].
BODIPY 665/676是一种亲脂性荧光探针，高亲和力结合游离脂肪酸和甘油三酯，具有长波长的最大吸收波长(λex=665nm)和最大荧光发射波长(Em=676nm)[1]。与常用的脂质过氧化探针C11 BODIPY 581/591(MX5211-1MG)相似，BODIPY 665/676容易被过氧化自由基氧化，一旦氧化，最大发射波长迁移到605nm(λex=580 nm) [2-4]。BODIPY 665/676最重要的优势在于发射波长是676nm，与绝大部分的荧光素之间无交叉，从而能避免比如自荧光的干扰。BODIPY 665/676具热稳定和高度疏水性特征，从而不会扩散出脂滴。激发器642nm与最大吸收波长665nm接近，由此，在很低的探针浓度下就能得到强荧光信号[5]。