BODIPY 630/650X succinimide ester is a fluorescent dye derivative characterized by a high fluorescence quantum yield and far-red emission (ex/em=646/660nm). BODIPY 630/650X succinimide ester achieves specific labeling by covalently reacting with the primary amines of biological molecules, such as proteins, via its succinimidyl ester group. BODIPY 630/650X succinimide ester can be utilized in research related to cell imaging, protein tracking, and molecular probe construction[1].
References:
[1] Goulding J, Mistry SJ, Soave M, et al. Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2-adrenoceptor in CRISPR/Cas9 genome-edited HEK293T cells at low expression levels. Pharmacol Res Perspect. 2021 May;9(3):e00779.
[2] Baker JG, Adams LA, Salchow K, et al. Synthesis and characterization of high-affinity 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled fluorescent ligands for human β-adrenoceptors. J Med Chem. 2011 Oct 13;54(19):6874-87.
[3] Lo CL, Yip SP, Cheng PK, et al. One-step rapid reverse transcription-PCR assay for detecting and typing dengue viruses with GC tail and induced fluorescence resonance energy transfer techniques for melting temperature and color multiplexing. Clin Chem. 2007 Apr;53(4):594-9.
[4] Bedner E, Ruan Q, Chen S, et al. Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry. 2000 Aug 1;40(4):271-9.
BODIPY 630/650X succinimide ester是一种荧光染料衍生物，具有高荧光量子产率和远红光发射特性（ex/em=646/660nm）。BODIPY 630/650X succinimide ester可通过其succinimide ester基团与蛋白质等生物分子的伯氨基发生共价反应以实现特异性标记。BODIPY 630/650X succinimide ester可用于细胞成像、蛋白质追踪和分子探针构建的相关研究[1-4]。