BODIPY 576/589 is a red-fluorescent labeling dye with distinctive hydrophobic properties that make BODIPY 576/589 ideal for tagging lipids, membranes, and other lipophilic compounds, BODIPY 576/589 absorption and emission maxima are 576nm and 589nm, respectively, and its extinction coefficient is 83000cm⁻¹ M⁻¹. The dye features a high extinction coefficient and fluorescence quantum yield, and BODIPY 576/589 remains relatively insensitive to pH changes and solvent polarity. Owing to its comparatively long excited-state lifetime, BODIPY 576/589 is suitable for fluorescence-polarization–based assays and exhibits a large two-photon cross-section for multiphoton excitation; BODIPY 576/589 is activated to label proteins, DNA, cells, etc.
References:
[1] Winter S, Kirschstein S, Miller DP. Molecular dynamics of labeled oligonucleotide probes used for the detection of point mutations in DNA. Nucleosides Nucleotides. 1999 Mar;18(3):411-23.
[2] Das S, Dey S, Patra S, et al. BODIPY-Based Molecules for Biomedical Applications. Biomolecules. 2023 Nov 30;13(12):1723.
BODIPY 576/589是一种红色荧光标记染料，具有独特的疏水特性，适用于标记脂质、膜或其他亲脂性化合物，最大激发和发射波长为576nm和589nm，消光系数为83000cm⁻¹M⁻¹。BODIPY 576/589具有高消光系数和荧光量子产率，对pH值变化和溶剂极性相对不敏感。BODIPY 576/589染料具有相对较长的激发状态寿命，可用于基于荧光偏振的测定以及多光子激发的大型双光子横截面，也可用于基于荧光寿命测量的各种方法中。BODIPY 576/589经过活化，可以标记蛋白质、DNA以及细胞等。